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Molecular characterization of Cryptosporidium spp. in native calves in Nigeria

Abstract

Most studies on the distribution of Cryptosporidium species in cattle were done with dairy breeds in industrialized nations. In this study, 65 fecal samples from randomly selected 12–24-week-old diarrheic calves in four white Fulani herds in southwestern Nigeria were screened for Cryptosporidium spp. using polymerase chain reaction (PCR)–restriction fragment length polymorphism (RFLP) analysis of the small subunit rRNA gene. Thirty-four (52.3%) of the samples were positive for Cryptosporidium. RFLP analysis of PCR products showed that 18 (27.7%) and five (7.7%) of the positive samples had Cryptosporidium bovis and Cryptosporidium ryanae, respectively, and 11 (16.9%) had mixed infections of the two species. The absence of C. parvum suggests that the age group of calves studied is not likely to be source of zoonotic infection to humans.

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Acknowledgements

This work was supported by the John D. and Catherine T. MacArthur Foundation Overseas Training Grant from University of Ibadan (Nigeria) and the Center for Diseases Control and Prevention, Atlanta, GA, USA. We thank Theresa Dearen for technical assistance. The findings and conclusions in this report are those of the authors and do not necessarily represent the views of the Centers for Disease Control and Prevention.

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Correspondence to Lihua Xiao.

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Ayinmode, A.B., Olakunle, F.B. & Xiao, L. Molecular characterization of Cryptosporidium spp. in native calves in Nigeria. Parasitol Res 107, 1019–1021 (2010). https://doi.org/10.1007/s00436-010-1972-1

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Keywords

  • Restriction Fragment Length Polymorphism
  • Restriction Fragment Length Polymorphism Analysis
  • Cryptosporidiosis
  • Restriction Fragment Length Polymorphism Pattern
  • Santin