Advertisement

Springer Nature is making Coronavirus research free. View research | View latest news | Sign up for updates

The DNA-induced protective immunity with chicken interferon gamma against poultry coccidiosis

  • 256 Accesses

  • 22 Citations

Abstract

The immunogenicity of Eimeria acervulina cSZ-2 and chicken interferon gamma was observed against Eimeria tenella challenge. The chickens were randomly divided into six groups of 24 chicks each. Three groups of chickens were injected with DNA vaccines pVAX1-cSZ2, pVAX1-chIFN-γ and pVAX1-cSZ2-chIFN-γ two times (at days 14 and 21) at a dose of 100 µg intramuscularly. Three other groups were kept as control and injected with TE buffer (10 mM Tris–HCl pH 7.6 and 1 mM EDTA). One week following the booster dose, all chickens except the non-infected, non-vaccinated control group were inoculated orally with 5 × 104 sporulated oocysts of E. tenella. Seven days post challenge, all chickens were weighted and slaughtered for cecal lesion scoring and oocyst counts. The results demonstrated that cSZ-2 in combination with interferon gamma can protect chickens from coccidiosis by significantly decreasing body weight loss and oocyst excretion reflecting partial protection against E. tenella infection, and further studies are necessary to test for protection against other Eimeria species.

This is a preview of subscription content, log in to check access.

References

  1. Hilton LS, Bean AG, Lowenthal JW (2002) The emerging role of avian cytokines as immunotherapeutics and vaccine adjuvants. Vet Immunol Immunopathol 85:119–128

  2. Kim JJ, Simbiri KA, Sin JI, Dang K, Oh J, Dentchev T et al (1999) Cytokine molecular adjuvants modulate immune responses induced by DNA vaccine constructs for HIV-1 and SIV. J Interferon Cytokine Res 19:77–84

  3. Laurent F, Bourdieu C, Kazanji M, Yvore P, Pery P (1994) The immunodominant Eimeria acervulina sporozoite antigen previously described as p160/p240 is a 19-kilodalton antigen present in several Eimeria species. Mol Biochem Parasitol 63:79–86

  4. Long PL, Reid WM (1982) A guide for the diagnosis of coccidiosis in chickens. The University of Georgia, College of Agriculture Experiment Stations. Research report 404:1–17

  5. Li GQ, Kanu S, Xiao SM, Xiang FY (2005) Responses of chickens vaccinated with a live attenuated multi-valent ionophore-tolerant Eimeria vaccine. Vet Parasitol 129:179–186

  6. Lillehoj HS, Choi KD (1998) Recombinant chicken interferon-γ-mediated inhibition of Eimeria tenella development in vitro and reduction of oocyst production and body weight loss following Eimeria acervulina challenge infection. Avian Dis 42:307–314

  7. Lillehoj HS, Ding X, Quiroz MA, Bevensee E, Lillehoj EP (2005) Resistance to intestinal coccidiosis following DNA immunization with the cloned 3-1E Eimeria gene plus IL-2, IL-15, and IFN-gamma. Avian Dis 49:112–117

  8. Maecker HT, Umetsu DT, DeKruyff RH, Levy S (1997) DNA vaccination with cytokine fusion construct biases the immune response to ovalbumin. Vaccine 15:1687–1696

  9. Min W, Lillehoj HS, Burnside J, Weining KC, Staeheli P, Zhu JJ (2002) Adjuvant effects of IL-1beta, IL-2, IL-8, IL-15, IFN-alpha, IFN-gamma TGF-beta4 and lymphotactin on DNA vaccination against Eimeria acervulina. Vaccine 20:267–274

  10. Peek HW, Landman WJM (2003) Resistance to anticoccidial drugs of Dutch avian Eimeria spp. Field isolates originating from 1996, 1999 and 2001. Avian Pathol 32:391–401

  11. Shah MAA, Xu L, Yan R, Song X, Li XR (2010) Cross immunity of DNA vaccine pVAX1-cSZ2-IL-2 to Eimeria tenella, E. necatrix and E. maxima. Exp Parasitol 124:330–333

  12. Shah MAA, Xu L, Yan R, Song X, Li XR (2010b) Construction of DNA vaccines encoding Eimeria acervulina cSZ-2 with chicken IL-2 and IFN-γ and their induced immunity against poultry coccidiosis. Res Vet Sci doi:10.1016/j.rvsc.2010.05.003

  13. Song KD, Lillehoj HS, Choi KD, Yun CH, Parcells MS, Huynh JT et al (2000) A DNA vaccine encoding a conserved Eimeria protein induces protective immunity against live Eimeria acervulina challenge. Vaccine 19:243–52

  14. Song XK, Xu L, Yan R, Huang X, Shah M, Ali A, Li XR (2009) The optimal immunization procedure of DNA vaccine pcDNA-TA4-IL-2 of Eimeria tenella and its cross immunity to Eimeria necatrix and Eimeria acervulina. Vet Parasitol 159:30–36

  15. Takehara K, Kobayashi K, Ruttanapumma R, Kamikawa M, Nagata T, Yokomizo Y, Nakamura M (2003) Adjuvant efffect of chicken interferon gammma for inactivated Salmonella enteritidis antigen. J Vet Med Sci 65:1337–1341

  16. Xu SZ, Chen T, Wang M (2006) Protective immunity enhanced by chimeric DNA prime-protein booster strategy against Eimeria tenella challenge. Avian Dis 50:579–85

  17. Xu Q, Song X, Xu L, Yan R, Shah MAA, Li XR (2008) Vaccination of chickens with a chimeric DNA vaccine encoding Eimeria tenella TA4 and chicken IL-2 induces protective immunity against coccidiosis. Vet Parasitol 156:319–323

  18. Williams RB (2002) Anticoccidial vaccines for broiler chickens: pathways to success. Avian Pathol 31:317–53

  19. Wu SQ, Wang M, Liu Q, Zhu YJ, Suo X, Jiang JS (2004) Construction of DNA vaccines and their induced protective immunity against experimental Eimeria tenella infection. Parasitol Res 9:332–336

Download references

Acknowledgements

This work was supported by the National Hi-Tech Research and Development Program of China (863 program) under grant number 2006AA10A207. The author Muhammad Ali A. Shah was supported by Higher Education Commission (HEC), Pakistan, against PhD overseas scholarship scheme.

Author information

Correspondence to Xiangrui Li.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Shah, M.A.A., Song, X., Xu, L. et al. The DNA-induced protective immunity with chicken interferon gamma against poultry coccidiosis. Parasitol Res 107, 747–750 (2010). https://doi.org/10.1007/s00436-010-1940-9

Download citation

Keywords

  • Coccidiosis
  • Eimeria Species
  • Sporulated Oocyst
  • Oocyst Excretion
  • Potassium Dichromate Solution