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Efficacy of Chrysosporium tropicum metabolite against mixed population of adult mosquito (Culex quinquefasciatus, Anopheles stephensii, and Aedes aegypti) after purification with flash chromatography

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Abstract

Chrysosporium tropicum is a keratinophilic fungus and an effective mosquito control agent. This fungus was grown in Richards broth at 27 ± 2°C and a relative humidity of 75% ± 5% for 15 ± 2 days. Filtration was done with Whatman number 1 filter paper, column chromatography, and flash chromatography. Adulticidal efficacy was performed against a mixed population of mosquitoes including Culex quinquefasciatus, Anophelese stephensii, Aedes aegypti at five different concentrations 5:5, 6:4, 7:3, 8:2, 9:1 by adding fungal filtrate of flash chromatography to methanol in different ratio (metabolite/methanol). The experiment was conducted in the cage with an area of 2 × 2 × 3 ft. The mortality in mosquito population was recorded after 8 hours of exposure, and adulticidal activity was tested by probit analysis. The LC50 was determined to be 4.9921 ml. Results of present study confirm that metabolites of C. tropicum can be utilized as alternative biological control agents for adult mosquitoes.

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Acknowledgements

We sincerely wish to acknowledge Prof. V. G.Das, Director, Dayalbagh Educational Institute for the facilities provided and Prof. K.K. Dua, Head, Department of Zoology for providing the opportunity and facilities to complete work.

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Correspondence to Preeti Verma.

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Verma, P., Prakash, S. Efficacy of Chrysosporium tropicum metabolite against mixed population of adult mosquito (Culex quinquefasciatus, Anopheles stephensii, and Aedes aegypti) after purification with flash chromatography. Parasitol Res 107, 163–166 (2010). https://doi.org/10.1007/s00436-010-1854-6

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Keywords

  • Instar Larva
  • Entomopathogenic Fungus
  • Lymphatic Filariasis
  • Mosquito Larva
  • Flash Chromatography