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Efficacy of Chrysosporium tropicum metabolite against mixed population of adult mosquito (Culex quinquefasciatus, Anopheles stephensii, and Aedes aegypti) after purification with flash chromatography

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Chrysosporium tropicum is a keratinophilic fungus and an effective mosquito control agent. This fungus was grown in Richards broth at 27 ± 2°C and a relative humidity of 75% ± 5% for 15 ± 2 days. Filtration was done with Whatman number 1 filter paper, column chromatography, and flash chromatography. Adulticidal efficacy was performed against a mixed population of mosquitoes including Culex quinquefasciatus, Anophelese stephensii, Aedes aegypti at five different concentrations 5:5, 6:4, 7:3, 8:2, 9:1 by adding fungal filtrate of flash chromatography to methanol in different ratio (metabolite/methanol). The experiment was conducted in the cage with an area of 2 × 2 × 3 ft. The mortality in mosquito population was recorded after 8 hours of exposure, and adulticidal activity was tested by probit analysis. The LC50 was determined to be 4.9921 ml. Results of present study confirm that metabolites of C. tropicum can be utilized as alternative biological control agents for adult mosquitoes.

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  1. Ponlawat A, Scott JG, Harrington LC (2005) Insecticide susceptibility of Aedes aegypti & Aedes albopictus across Thailand. J. Med Entomol 42(5):821–825

  2. Amer A, Mehlhorn H (2006) Persistency of larvicidal effects of plant oil extracts under different storage conditions. Parasitol Res 99:473–477

  3. Balaramn K, Bheema Rao US, Rajagopalan PK (1979) Isolation of Metarhizium anisopliae, Beauveria tenella and Fusarium oxysporum (Deuteromycetes) and their pathogenicity to Culex fatigans and Anopheles stephensi. Indian J Med Res 70:718–722

  4. Federici BA (1981) (555–572) Mosquito control by fungi Culicinomyces, Leginidium & Coelomomyces. In: Microbial Control of Pests & Plant Diseases 1970–1980 (ed.). Academic, New York, pp 555–572

  5. Finney DJ (1971) Probit analysis, 3rd edn. Cambridge University Press, Cambridge, UK, Cambridge United Kingdom

  6. Govindrajan M, Jebanesan A, Reetha D (2005) Larvicidal effect of extracellular secondary metabolites of different fungi against the mosquito, Culex quinquefasciatus. Trop Biomed 22(1):1–3

  7. Hargreaves K, Koekemoer LL, Brooke BD, Hunt RH, Methembe J, Coetzee M (2000) Anopheles funestus resistant to pyrethroid insecticides in South Africa. Med Vet Entomol 2:181–189

  8. James RR, Lighthart B (1994) Susceptibility of the convergent lady beetle to four entomopathogenic fungi. Environ Entomol 23:188–190

  9. Krischbam JB (1985) Potential implication of genetic engineering and other biotechnologies to insect control. Annu Rev Entomol 30:51–70

  10. Lecy CM, Lacey LA, Roberts DR (1988) Route of invasion and histopathology of Metarhizium anisopliae in Culex quinquefasciatus. J Invertebr Pathol 52:108–118

  11. Legner EF (1995) Biological control of diptera of medical and veterinary importance. J Vector Ecol 20:59–120

  12. McCray EM Jr, Womelderoff DJ, Husbands RC, Eliason DA (1973) Laboratory observation and field tests with Legenidium against California Mosquitoes. Proc Cali Mosq Control Assoc 41:123–128

  13. Michaelakins A, Strongilos AT, Bouzas EA, Koliopoulos G, Elias A (2008) Couladouros larvicidal activity of naturally occurring naphthoquinones and derivatives against the West Nile virus vector Culex pipiens. Parasitol Res 104:657–662

  14. Mohanty SS, Prakash S (2007) Efficacy of Chrysosporium lobatum against larvae of malaria vector, Anopheles stephensi in the laboratory. Curr Sci 83:1585–1588

  15. Mohanty SS, Prakash S (2008) Laboratory and field evaluation of the fungus Chrysosporium lobatum against larvae of mosquito Culex quinquefasciatus. Parasitol Res 102:881–886

  16. Mohanty SS, Raghvendra K, Rai U, Dash PA (2008) efficacy of female Culex quinquefasciatus with entomopathogenic fungus Fusarium pallidoroseum. Parasitol Res 103:171–174

  17. Priyanka and Prakash (2001) Chrysosporium tropicum efficacy against Anophelese stephensi larvae in the laboratory. J Am Mosq Control Assoc 17:127–130

  18. Rozendaal JA (1997) vector control, methods for use by individuals and communities. World Health organization, Geneva, Switzerland, pp 7–177

  19. Scholte E-J, Knols BGJ, Samson RA, Takkan W (2004) Entomopathogenic fungi for mosquito control: a review. J Insect Sci 4:19

  20. Strasser H, Vey A, Butt TM (2000) Are there any risk in using entomopathogenic fungi for pest control, with particular reference to the bio active metabolites of Metarhizium tolipocladium & Beauveria species? Biocontrol Sci & Tech 10:717-735

  21. Vyas N, Dua K, Prakash S (2007) Efficacy of Lagenidium giganteum metabolites on mosquito larvae with reference to nontarget organisms. Parasitol Res 101:385–390

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We sincerely wish to acknowledge Prof. V. G.Das, Director, Dayalbagh Educational Institute for the facilities provided and Prof. K.K. Dua, Head, Department of Zoology for providing the opportunity and facilities to complete work.

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Correspondence to Preeti Verma.

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Verma, P., Prakash, S. Efficacy of Chrysosporium tropicum metabolite against mixed population of adult mosquito (Culex quinquefasciatus, Anopheles stephensii, and Aedes aegypti) after purification with flash chromatography. Parasitol Res 107, 163–166 (2010).

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  • Instar Larva
  • Entomopathogenic Fungus
  • Lymphatic Filariasis
  • Mosquito Larva
  • Flash Chromatography