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Molecular and experimental evidence refuse the life cycle of Proctoeces lintoni (Fellodistomidae) in Chile

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Abstract

The mussel Perumitylus purpuratus has been described as the first intermediate host for the digenea Proctoeces lintoni (Fellodistomidae) in the Chilean coast. The study of more than 3000 specimens of P. purpuratus, taken off northern Chile revealed the absence of sporocysts. Experimental infection of mussels with eggs obtained from the known host for P. lintoni was unsuccessful. We analyze the V4 region of the SSU rRNA of living sporocysts and cercariae obtained from P. purpuratus from central Chile in order to confirm the proposed life cycle for this digenea. Our results demonstrated that sporocysts and cercariae obtained from P. purpuratus do not belong to P. lintoni but to an undescribed digenea from the Chilean coast.

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Acknowledgments

This study was supported by Fondo Nacional de Ciencia y Tecnologia (National Fund for Science and Technology) FONDECYT-Chile Grant 1070898 to MEO.

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Correspondence to Marcelo E. Oliva.

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Oliva, M.E., Valdivia, I.M., Cárdenas, L. et al. Molecular and experimental evidence refuse the life cycle of Proctoeces lintoni (Fellodistomidae) in Chile. Parasitol Res 106, 737–740 (2010). https://doi.org/10.1007/s00436-009-1708-2

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Keywords

  • Intermediate Host
  • Random Sequence Addition
  • Accession Number AJ224461
  • Bayesian Phylogenetic Analysis
  • Chilean Coast