Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Molecular identification of Phortica variegata and Phortica semivirgo (Drosophilidae, Steganinae) by PCR-RFLP of the mitochondrial cytochrome oxidase c subunit I gene

This is a preview of subscription content, log in to check access.

Fig. 1


  1. Armstrong KF, Ball SL (2005) DNA barcodes for biosecurity: invasive species identification. Philos Trans R Soc Lond B Biol Sci 360:1813–1823 Erratum in: Philos Trans R Soc Lond B Biol Sci 360:2373

  2. Bächli G, Vilela CR, Andersson Esher S, Saura A (2005) The Drosophilidae (Diptera) of Fennoscandia and Denmark, vol. 39. Fauna Entomologica Scandinavica, Brill, Leiden

  3. Lunt DH, Zhang DX, Szymura JM, Hewitt GM (1996) The insect cytochrome oxidase I gene: evolutionary patterns and conserved primers for phylogenetic studies. Insect Mol Biol 5:153–165

  4. Máca J (1977) Revision of Palearctic species of Amiota subg. Phortica (Diptera, Drosophilidae). Acta Entomologica Bohemoslovaca 74:115–130

  5. Máca J (1987) Amiota (Phortica) goetzi sp. n. (Diptera, Drosophilidae) with faunistic notes to Drosophilidae, Odiniidae and Periscelididae from southeastern Europe and Turkey. Acta Ent Mus Natn Pragae 42:311–320

  6. Navajas M, Fenton B (2000) The application of molecular markers in the study of diversity in acarology: a review. Exp Appl Acarol 24:751–774

  7. Otranto D, Stevens JR (2002) Molecular approaches to the study of myiasis-causing larvae. Int J Parasitol 32:1345–1360

  8. Otranto D, Traversa D, Guida B, Tarsitano E, Fiorente P, Stevens JR (2003) Molecular characterization of the mitochondrial cytochrome oxidase I gene of Oestridae species causing obligate myiasis. Med Vet Entomol 17:307–315

  9. Otranto D, Lia RP, Cantacessi C, Testini G, Troccoli A, Shen JL, Wang ZX (2005) Nematode biology and larval development of Thelazia callipaeda (Spirurida, Thelaziidae) in the drosophilid intermediate host in Europe and China. Parasitology 131:847–855

  10. Otranto D, Brianti E, Cantacessi C, Lia RP, Máca J (2006a) The zoophilic fruit fly Phortica variegata: morphology, ecology and biological niche. Med Vet Entomol 20:358–364

  11. Otranto D, Cantacessi C, Testini G, Lia RP (2006b) Phortica variegata is an intermediate host of Thelazia callipaeda under natural conditions: evidence for pathogen transmission by a male arthropod vector. Int J Parasitol 36:1167–1173

  12. Otranto D, Cantacessi C, Mallia E, Lia RP (2007) First report of Thelazia callipaeda (Spirurida, Thelaziidae) in wolves in Italy. J Wildl Dis 43:518–520

  13. Otranto D, Stevens JR, Testini G, Cantacessi C, Máca J (2008) Molecular characterization and phylogenesis of Steganinae (Diptera, Drosophilidae) inferred by the mitochondrial cytochrome c oxidase subunit I. Med Vet Entomol 22:37–47

  14. Tamura K, Dudley J, Nei M, Kumar S (2007) MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol 24:1596–1599

  15. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 24:4876–4882

  16. Zhang DX, Hewitt GM (1996) Assessment of the universality and utility of a set of conserved mitochondrial COI primers in insects. Insect Mol Biol 6:143–150

Download references

Author information

Correspondence to Domenico Otranto.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Cantacessi, C., Traversa, D., Testini, G. et al. Molecular identification of Phortica variegata and Phortica semivirgo (Drosophilidae, Steganinae) by PCR-RFLP of the mitochondrial cytochrome oxidase c subunit I gene. Parasitol Res 103, 727–730 (2008).

Download citation


  • Polymerase Chain Reaction Product
  • Morphological Identification
  • Restriction Profile
  • Cox1 Sequence
  • Unequivocal Identification