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Sarcocystis sp. from the goldeneye (Bucephala clangula) and the mallard (Anas platyrhynchos): cyst morphology and ribosomal DNA analysis

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Abstract

By light microscopy, cysts of Sarcocystis sp. (cyst type I) from the goldeneye (Bucephala clangula) seemed filamentous with a smooth and thin (<1 μm) cyst wall. Ultrastructurally, the cyst wall surface was irregular with minute undulations of the primary cyst wall. These sarcocysts had type-1 cyst wall. Cystozoites were banana-shaped and measured 7.0–8.5 μm in length. By light microscopy, cysts of Sarcocystis sp. (cyst type II) from the mallard (Anas platyrhynchos) were ribbon-shaped, very long, and thin. On the surface of the wall (up to 1.5 μm), they had palisade-like villar protrusions closely crowded together. Electron micrographs showed villar protrusions (up to 1.3 μm in length) different in size and shape. The latter had short microprojections especially obvious in the oblique sections. Cystozoites were slightly bent with blunt ends, broader at one end, and measured 13.0–16.1 × 1.8–2.5 μm. Phylogenetic analysis based on the comparison of partial 28S rRNR gene sequences of Sarcocystis sp. (cyst type II) derived from the mallard, Sarcocystis sp. (cyst type I) and Sarcocystis sp. (cyst type III) both derived from the white-fronted goose (Anser albifrons) suggested that these sequences belonged to separate Sarcocystis species.

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Acknowledgements

We would like to express our deep thanks to Professor Richard W. Ashford from the Liverpool School of Tropical Medicine for the valuable comments on an early draft of the paper and for correcting the English. The authors are grateful to Mrs. I. Žalakevičienė from the Institute of Experimental and Clinical Medicine for the help in carrying out investigations of electron microscopy.

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Correspondence to Dalius Butkauskas.

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Kutkienė, L., Sruoga, A. & Butkauskas, D. Sarcocystis sp. from the goldeneye (Bucephala clangula) and the mallard (Anas platyrhynchos): cyst morphology and ribosomal DNA analysis. Parasitol Res 102, 691–696 (2008). https://doi.org/10.1007/s00436-007-0813-3

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Keywords

  • Cyst Wall
  • Cyst Type
  • Sarcocystis Species
  • Southern High Plain
  • Wild Duck