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From molecule to diagnostic tool: Theileria annulata surface protein TaSP

Abstract

Isolation and characterization of the Theileria annulata macroshizont stage protein TaSP showed that this parasite surface membrane protein is a highly antigenic protein suitable for the development of diagnostic tools for tropical theileriosis. An enzyme-linked immunosorbent assay (ELISA) for the detection of circulating antibodies against Theileria annulata was established and validated using the recombinantly expressed TaSP protein. The ELISA has subsequently been applied for cross-sectional surveys to determine the distribution and prevalence of tropical theileriosis in Sudan.

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Acknowledgment

Supported in part by the European Commission’s Inco-Dev Research Program “Molecular and immunological characterization of merozoite antigens and their encoding genes of a Theileria species highly pathogenic for small ruminants in China: application for the development of diagnostics and vaccine,” (ADDAV), Project number ICA-1999-30151, and Coordinated Action “Integrated Consortium on Ticks and Tick-borne Diseases” (ICTTD-3), Project number 510561.

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Correspondence to Ulrike Seitzer.

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Seitzer, U., Bakheit, M.A., Salih, D.E.A. et al. From molecule to diagnostic tool: Theileria annulata surface protein TaSP. Parasitol Res 101, 217–223 (2007). https://doi.org/10.1007/s00436-007-0685-6

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Keywords

  • Babesia
  • Polymorphic Region
  • Indirect Fluorescence Antibody Test
  • Reverse Line Blot
  • Schizont Stage