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Parasitology Research

, Volume 101, Issue 5, pp 1225–1231 | Cite as

Molecular cloning and enzymatic characterization of a class mu glutathione S-transferase of Paragonimus westermani

  • Tae Yun Kim
  • Ji-Yun Lee
  • Tae Im Kim
  • Ki Ho Moon
  • Shin-Yong Kang
  • Sung-Jong HongEmail author
Original Paper

Abstract

Glutathione S-transferase (GST) is a component of a second line of defense against bioreactive radicals derived from host immune attack. Paragonimus westermani causes acute or chronic lung diseases in mammals. A cDNA clone, PwGST#11, of adult P. westermani produced in the present study was 748 bp long and encoded an open reading frame of 217 amino acids with a starting methionine. The molecular mass of this putative polypeptide, Pw26GST, was estimated to be 25.1 kDa with an isoelectric point of 5.7. Pw26GST was homologous with the 26-kDa GSTs of trematodes and vertebrates. Nine of the ten amino acid residues lining the glutathione-binding pocket were conserved. Putative Pw26GST polypeptide was clustered with 26-kDa GSTs of trematodes belonging to the class μ. Recombinant Pw26GST protein generated bacterially, revealed GST enzyme activity toward an universal and class μ-specific substrates. Mouse antisera to recombinant Pw26GST protein recognized native 26-kDa GST of P. westermani but not the GSTs of any other trematodes. Collectively, Pw26GST was found to be a member of class μ GSTs of P. westermani.

Keywords

cDNA Fragment CDNB Conserve Amino Acid Residue Immune Seron Degenerate Oligonucleotide Primer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Tae Yun Kim
    • 2
  • Ji-Yun Lee
    • 1
  • Tae Im Kim
    • 1
  • Ki Ho Moon
    • 1
  • Shin-Yong Kang
    • 1
  • Sung-Jong Hong
    • 1
    Email author
  1. 1.Department of ParasitologyChung-Ang University College of MedicineSeoulSouth Korea
  2. 2.Department of Infection Biology, College of MedicineChungnam National UniversityDaejeonSouth Korea

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