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Serum protein alterations in dogs naturally infected with Toxoplasma gondii

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Abstract

We conducted this study to describe the serum electrophoretic pattern in dogs associated with the infection of Toxoplasma gondii (T. gondii). The serum protein pattern of 25 dogs with confirmed T. gondii infection and 15 clinically healthy dogs were evaluated using native polyacrylamide gel electrophoresis. Albumin, alpha-1 globulin, alpha-2 globulin, beta globulin, and gamma globulin bands were seen from the serum electrophoresis of infected and healthy dogs. Compared to the control group, significant decreases in the mean percentages of albumin (from 46.1 ± 7.2 to 40.8 ± 4.5%, P < 0.05), alpha-1 globulin (from 3.9 ± 0.4 to 0.8 ± 0.2%, P < 0.001), alpha-2 globulin (from 9.0 ± 0.4 to 8.3 ± 0.8%, P < 0.01), and beta globulin (from 18.4 ± 1.2 to 12.1 ± 0.6%, P < 0.001) in the infected group were determined. In contrast, gamma globulin fraction was significantly higher in infected dogs (38.1 ± 4.6%) than in control dogs (22.7 ± 7.2%; P < 0.001). Moreover, significant correlations were determined between the percentages of the albumin and gamma globulin fractions and liver enzyme tests including aspartate aminotransferase and alanine aminotransferase in infected dogs; however, no correlation was observed for the other protein fractions. In conclusion, marked alterations in serum protein pattern associated with strong modifications of serum protein concentrations are in accordance with the hepatic injury as affirmed by liver enzyme tests that were demonstrated in the canine toxoplasmosis. These findings showed that serum protein electrophoresis can be used in the diagnosis and prognosis of canine toxoplasmosis as a supplementary analysis in combination with serological, clinical, and laboratory findings of this disease.

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Acknowledgment

The authors thank to Dr. Safa Gurcan, Department of Biostatistic, Faculty of Veterinary Medicine, Ankara University, for the statistic analysis. We would also like to thank Dr. Erol Handemir and Dr. Kadir Kamburgil for IFAT test, Veterinary Control and Research Institute, 42080, Konya, Turkey.

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Correspondence to Gul Fatma Yarim.

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Yarim, G.F., Nisbet, C., Oncel, T. et al. Serum protein alterations in dogs naturally infected with Toxoplasma gondii . Parasitol Res 101, 1197–1202 (2007). https://doi.org/10.1007/s00436-007-0601-0

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Keywords

  • Beta Globulin
  • Toxoplasmosis
  • Infected Group
  • Gamma Globulin
  • Babesia