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Preliminary protective capacity study of a Dicrocoelium dendriticum antigenic protein in hamsters

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Abstract

The purpose of this study was to evaluate the protective capacity of 130 kDa Dicrocoelium dendriticum protein in hamsters experimentally infected with this parasite. Forty hamsters divided into four groups of ten animals each were used: G1 (control), G2 (infected), G3 (immunized with Freund’s adjuvant and infected), G4 (130 kDa protein vaccinated + adjuvant and infected). Infection with 40 metacercariae/hamster was carried out 4 weeks after the last immunization. Parasitological studies [number of eggs per gram (epg) and worm burden] and biochemical parameters (total proteins, albumin, and total bilirubin), hepatic enzymes [aspartate aminotransferase (AST) and alanine aminotransferase (ALT)], and total IgG levels were determined. A reduction in epg in G3 and G4 was observed 16 weeks postinfection with the higher reduction percentage in the latter (25.2%). No statistically significant differences were detected in the number of recovered worms among groups, although the mean was slightly less in G4 (12.2±2.08, mean±SE) than in G2 (15.4±2.90). In G4, global protection was 20.9% and an increase in AST and ALT levels was observed. Total IgG levels were similar in the three infected groups. The protection obtained was inadequate, so the antigen dose, immunization–infection period, adjuvants, and immunization route must be optimized.

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References

  1. Campo R, Manga-González MY, González-Lanza C (2000) Relationship between egg output and parasitic burden in lambs experimentally infected with different doses of Dicrocoelium dendriticum (Digenea). Vet Parasitol 87:139–149

  2. Casanueva P, Hillyer GV, Ramajo V, Oleaga A, Espinoza EY, Muro A (2001) Immunoprophylaxis against Fasciola hepatica in rabbits using a recombinant Fh15 fatty acid-binding protein. J Parasitol 87:697–700

  3. Dalton JP, Mulcahy G (2001) Parasite vaccines—a reality? Vet Parasitol 98:149–167

  4. Espino AM, Gil R, Osuna A, Hillyer GV (2005) Fasciola hepatica: humoral and cytokine responses to a member of the saponin-like protein family following delivery as a DNA vaccine in mice. Exp Parasitol 110:374–383

  5. González-Lanza C, Manga-González MY, Campo R, Del-Pozo P, Sandoval H, Oleaga A, Ramajo V (2000) IgG antibody response to ES or somatic antigens of Dicrocoelium dendriticum (Trematoda) in experimentally infected sheep. Parasitol Res 86:472–479

  6. Hillyer GV (2005) Fasciola antigens as vaccines against fascioliasis and schistosomiasis. J Helminthol 79:241–247

  7. López-Abán J, Oleaga A, Ramajo V, Casanueva P, Pérez Arellano JL, Hillyer GV, Muro A (2000) Vaccination of mice against Schistosoma bovis with a recombinant fatty acid binding protein from Fasciola hepatica. Vet Parasitol 91:33–42

  8. Manga-González MY, González-Lanza C, Cabanas E, Campo R (2001) Contributions to and review of dicrocoeliosis, with special reference to the intermediate hosts of Dicrocoelium dendriticum. Parasitology 123:S91–S114

  9. Manga-González MY, Ferreras MC, Campo R, González-Lanza C, Pérez V, García-Marín JF (2004) Hepatic marker enzymes, biochemical parameters and pathological effects in lambs experimentally infected with Dicrocoelium dendriticum (Digenea). Parasitol Res 93:344–355

  10. Martínez-Fernández AR, Nogal-Ruíz JJ, López-Abán J, Ramajo V, Oleaga A, Manga-González Y, Hillyer GV, Muro A (2004) Vaccination of mice and sheep with Fh12 FABP from Fasciola hepatica using the new adjuvant/immunomodulator system ADAD. Vet Parasitol 126:287–298

  11. Muro A, Ramajo V, López J, Simón F, Hillyer GV (1997) Fasciola hepatica: vaccination of rabbits with native and recombinant antigens related to fatty acid binding proteins. Vet Parasitol 69:219–229

  12. Otranto D, Traversa D (2002) A review of dicrocoeliosis of ruminants including recent advances in the diagnosis and treatment. Vet Parasitol 107:317–335

  13. Prokopič J, Kudrna K (1989) Juhok vakcinázása Dicrocoelium dendriticum ellen. Magy Allatorv L 44:405–406

  14. Ramajo V, Oleaga A, Casanueva P, Hillyer GV, Muro A (2001) Vaccination of sheep against Fasciola hepatica with homologous fatty acid binding proteins. Vet Parasitol 97:35–46

  15. Revilla-Nuín B, Manga-González MY, Miñambres B, González-Lanza C (2005) Partial characterization and isolation of 130 kDa antigenic protein of Dicrocoelium dendriticum adults. Vet Parasitol 134:229–240

  16. Sánchez-Campos S, Tuñón MJ, González P, Campo R, Ferreras MC, Manga-González MY, González-Gallego J (1996) Effects of experimental dicrocoeliosis on oxidative drug metabolism in hamster liver. Comp Biochem Physiol C 115:55–60

  17. Spithill TW, Dalton JP (1998) Progress in development of liver fluke vaccines. Parasitol Today 14:224–228

  18. Spithill TW, Smooker PM, Sexton JL, Bozas E, Morrison CA, Creaney J, Parsons JC (1999) Development of vaccines against Fasciola hepatica. In: Dalton JP (ed) Fasciolosis. CAB International, Wallingford, Oxon, UK, pp 377–410

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Acknowledgements

We wish to express our deep gratitude to M. P. Del-Pozo, M. L. Carcedo, and C. Espiniella (CSIC, León) for their technical assistance. We also thank Dr. C. C. Pérez-García, Director of the León University Animal Housing Section, for providing access to the facilities and L. Alaiz for helping in taking care of and handling the animals. This study was supported by the Spanish CICYT (Project No. AGL2001-1290). The experiments in this study comply with the current laws of the country where they were carried out (Spain).

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Correspondence to C. González-Lanza.

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González-Lanza, C., Manga-González, M.Y. & Revilla-Nuín, B. Preliminary protective capacity study of a Dicrocoelium dendriticum antigenic protein in hamsters. Parasitol Res 99, 749–752 (2006). https://doi.org/10.1007/s00436-006-0230-z

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Keywords

  • Adult Worm
  • Infected Group
  • Protective Capacity
  • Antigen Dose
  • Infected Control Group