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A novel genotype of Cryptosporidium muris from large Japanese field mice, Apodemus speciosus

Abstract

Cryptosporidium muris-like oocysts were isolated from large Japanese field mice, Apodemus speciosus. Morphologically, these oocysts resembled those obtained from a C. andersoni Kawatabi isolate but were smaller in size than those from a C.muris isolate. Following oral inoculation of the oocysts into large Japanese field mice and SCID mice, developing stages were found in the stomach epithelium. The infectivity of the isolate to wild and laboratory mice was slightly different from that of C.muris. DNA sequences of the 18S ribosomal RNA (rRNA) gene of the isolate were not identical to those of any known Cryptosporidium spp.; however, phylogenetic analysis indicated that the isolate was a member of the C.muris cluster. Differences between the isolate and C. muris are not significant at this point; therefore, we propose that this isolate is a novel genotype of C.muris and denote it as C. muris Japanese field mouse genotype.

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Acknowledgements

We thank Dr. Tokuma Yanai and Dr. Shigeharu Thujioka for technical assistance at the Gifu University, Dr. Yoshihisa Suyama and Dr. Masaaki Satoh for helpful and stimulating discussions at Tohoku University, and Dr. Motohiro Iseki for providing oocysts of the C. muris RN66 and the C. parvum HNJ-1 strains used in the study at the Medical School of Kanazawa University.

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Correspondence to Yutaka Nakai.

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Hikosaka, K., Nakai, Y. A novel genotype of Cryptosporidium muris from large Japanese field mice, Apodemus speciosus. Parasitol Res 97, 373–379 (2005). https://doi.org/10.1007/s00436-005-1459-7

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Keywords

  • SCID Mouse
  • Field Isolate
  • Maximum Likelihood Tree
  • Cryptosporidium Oocyst
  • Cryptosporidium Species