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Validation of the indirect TaSP enzyme-linked immunosorbent assay for diagnosis of Theileria annulata infection in cattle

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Abstract

An ELISA based on a recombinant Theileria annulata surface protein (TaSP) was evaluated for detection of antibodies in sera from cattle exposed to tropical theileriosis in Sudan. The reference positive samples, used in this study, were from Theileria-infected populations and consisted of 80 cattle from an endemic area in Khartoum State, with high antibody titers in the indirect fluorescent antibody test (IFAT). The reference negative samples were taken from non-exposed populations and consisted of 120 cattle maintained under strict tick control at a commercial farm in Sudan. The cut-off value determined by Two-Graph Receiver-Operating Characteristic (TG-ROC) curves was set at 31.6%, based on the positive reference samples. Further diagnostic validation was performed, which consisted of the measurement of the area under the ROC (AUC) and by valid range proportion (VRP), which was 0.97 and 0.98 for the cut-off, respectively. There were no cross-reactions with antibodies raised against Babesia spp. It is concluded that the TaSP ELISA is a useful test for the diagnosis of T. annulata infection in cattle under field conditions.

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Acknowledgements

This research was partially supported by the International Atomic Energy Agency (IAEA) through the Project No. SUD/05/027 entitled “Control of Ticks and Tick-borne Diseases”. Additional support was provided by the European Union (INCO-Dev programme, contract number ICA4-CT-2000-300028). An additional support was provided by the ICTTD Concerted Action Project on Integrated Control of Ticks and Tick-borne Diseases, supported by the INCO-DC Program of the European Union under contract no. IC18-CT95-0009. We thank Dr. R. Hashemi-Fesharki, Head Department of Protozoology and Protozoa Vaccine, Razzi Institute, Iran for the kind provision of the positive control serum and Dr. E. D. M. ELAmin, CVRL, Sudan for his critical review of the manuscript. Our thanks are also extended to Dr. M. Greiner for his valuable suggestions and comments. This work is published by kind permission of the Director-General Animal Resources Research Corporation.

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Correspondence to Jabbar S. Ahmed.

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Omer E. Shariff Deceased.

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Salih, D.E.A., Ahmed, J.S., Bakheit, M.A. et al. Validation of the indirect TaSP enzyme-linked immunosorbent assay for diagnosis of Theileria annulata infection in cattle. Parasitol Res 97, 302–308 (2005). https://doi.org/10.1007/s00436-005-1431-6

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Keywords

  • Intermediate Range
  • Babesia
  • Indirect Fluorescent Antibody Test
  • Percentage Positivity
  • Gaussian Method