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Taeniid tapeworm responses to in vitro glucose

Abstract

Experimental taeniid strobilae from Taenia solium and T. crassiceps (WFU strain) were incubated for 0–72 h in 0, 5 or 20 mM glucose solutions and further exposed for 15 min to the gap junction fluorochrome Lucifer Yellow. Frozen sections were obtained from each worm and observed under an epifluorescent microscope. Worm sections from strobilae incubated with glucose, revealed intense fluorescence in the base of the tegumentary surface, suggesting that this tissue behaves as a gap junction complex. Fluorescence intensity differences between control worms not exposed to glucose and worms incubated with glucose, were highly significant. The results demonstrate that under in vitro conditions, glucose is taken up along the whole strobilar tegument in both taeniid species, suggesting, that although taeniids attached to the duodenum probably take up most of their nutrients directly from the mucosal wall, the capacity for absorbing glucose along the tegumentary surface is always active and may increase the survival capacity of these intestinal worms by promoting glucose absorption at other points in the intestinal lumen.

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Acknowledgements

The authors thank Dr. Horacio Merchant for preparation of the confocal microscope images and Biol. Mauricio Castañòn Arreola for help in analysis of fluorescence intensity. This work was supported by grant# IN238602 from PAPIIT, DGAPA, UNAM, Mexico. The experiments herein, comply with the current laws of Science and Technology in Mexico.

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Correspondence to Kaethe Willms.

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Willms, K., Presas, A.M.F., Jiménez, J.A. et al. Taeniid tapeworm responses to in vitro glucose. Parasitol Res 96, 296–301 (2005). https://doi.org/10.1007/s00436-005-1348-0

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Keywords

  • Parenchymal Tissue
  • Lucifer Yellow
  • Duodenal Wall
  • Fluorescent Pattern
  • Colloidal Gold Particle