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Infectivity of Blastocystis isolates from chickens, quails and geese in chickens

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Abstract

The infectivity of six Blastocystis isolates obtained from two domestic chickens, two Japanese quails and two domestic geese, were examined in 1-week-old male chicks. All six isolates were able to infect the chicks via the intracecal inoculation of 1×106 cells of cultured organisms. Since the infected chicks discharged many cysts in their feces, the infectivity of the concentrated cysts in chicks was compared among three isolates from different bird species. The CK86-1 and QQ93-3 isolates, which were obtained from a chicken and a quail, respectively, were successfully infected in chicks by orally inoculating with 1×102–1×106 cysts. On the other hand, the AC03-1 isolate from a goose required more cysts to infect the chicks, from 1×103 cysts to 1×106 cysts. In addition, when an uninfected normal chick was housed with five experimentally inoculated chicks with cysts of the QQ93-3 isolate, the normal chick became infected, indicating the fecal–oral transmission of the cyst form among the birds. These results show that the transmission of Blastocystis infection occurs easily between the same or different bird species. Therefore, the proposal of new Blastocystis species on the basis of different avian host species is problematic.

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Acknowledgments

We thank Dr M. Singh, of the National University of Singapore, for providing the Blastocystis G3 isolate from a goose. This work was supported by a grant from the Japan Society for the Promotion of Science to H.Y. (C-16590338). All the experiments described comply with the current laws of the country in which the experiments were performed.

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Correspondence to Hisao Yoshikawa.

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Tanizaki, A., Yoshikawa, H., Iwatani, S. et al. Infectivity of Blastocystis isolates from chickens, quails and geese in chickens. Parasitol Res 96, 57–61 (2005). https://doi.org/10.1007/s00436-005-1326-6

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Keywords

  • Bird Species
  • Japanese Quail
  • Cecum Content
  • Fecal Culture
  • Fresh Fecal Sample