Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Effects of hypotonicity on amino acid release in adult Echinostoma caproni as determined by thin layer chromatography

  • 25 Accesses

  • 3 Citations

Abstract.

Thin layer chromatography (TLC) was used to analyze the amino acids in worm incubates isotonic and hypotonic to the intestinal habitat of adult Echinostoma caproni and to analyze the free pool amino acids of these trematodes after incubation. Qualitative analysis revealed the presence of histidine, lysine, alanine, and proline in all samples of incubate and worm tissue. Quantification of histidine and lysine by TLC with densitometry gave mean concentrations of 24.1 µg histidine/g worm per ml incubate in Locke's solution and 195.0 µg lysine/g worm per ml incubate in deioinized (DI) water. Quantification of histidine and lysine in the worm tissue gave mean weight percents of 0.0587 and 0.0263, respectively, in worms incubated in Locke's solution and 0.0229 and 0.0163, respectively, for worms incubated in DI water. Our findings suggest that E. caproni adults may leak amino acids for osmoregulation in hypotonic environments.

This is a preview of subscription content, log in to check access.

Author information

Additional information

Electronic Publication

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Ponder, E.L., Fried, B. & Sherma, J. Effects of hypotonicity on amino acid release in adult Echinostoma caproni as determined by thin layer chromatography. Parasitol Res 89, 242–244 (2003). https://doi.org/10.1007/s00436-002-0760-y

Download citation

Keywords

  • Qualitative Analysis
  • Proline
  • Lysine
  • Thin Layer
  • Alanine