This study describes the architecture of the coenosteal network in an Indonesian Stylaster species investigated by means of the X-ray computed microtomography (μ-CT) technique. The 3D approach allowed to characterize all internal cavity structures: a network of thin canals, gastropores, dactylopores, and ampullae. The main feature highlighted by this reconstruction is a dense network of thin canals extended to the entire colony. This network gives rise to and surrounds each cyclosystem. Moreover, the 3D analysis made it possible to study the reciprocal relationship between adjacent cyclosystems and to hypothesize the growth process of the branches of Stylaster sp.: each new cyclosystem buds between the gastropore and the dactylopores of the last formed one. The dactylopores of each cyclosystem are partially derived from the precedent one and are partially newly formed. The thin canals enveloping the dactylopores are actively involved in both the formation of a new gastropore and in the re-establishment of the typical amount of dactylopores in each cyclosystem. This growth process was confirmed by the scanning electron microscopy (SEM) observations of apical cyclosystems of several specimens. Results indicate that the non-destructive X-ray μ-CT technique can be fruitfully applied to characterize the coenosteal structures of stylasterids allowing the repetitive study of a specimen by means of virtually infinite section planes and different kinds of analyses (e.g., channel width and porosity).
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Bouillon J, Gravili C, Pagès F, Gili JM, Boero F (2006) An introduction to hydrozoa. Mémoir Mus Natl Hist Nat 194
Broch H (1942) Investigations on Stylasteridae (Hydrocorals). Skr Norske Vidensk Akad I Mat-Naturv Klasse 3:1–113 6 pls, 38 figs
Brun F, Mancini L, Kasae P, Favretto S, Dreossi D, Tromba G (2010) Pore3D: a software library for quantitative analysis of porous media. Nucl Instrum Meth A 615(3):326–332
Cairns SD (1983) A generic revision of the Stylasterina (Coelenterata: Hydrozoa). Part 1. Description of the genera. Bull Mar Sci 33:427–508
Cairns SD (1984) A generic revision of the Stylasteridae (Coelenterata: Hydrozoa). Part 2. Phylogenetic analysis. Bull Mar Sci 35:38–53
de Pourtalès LF (1871) Deep-sea corals. Illustr Cat Mus Comp Zool Harv Coll 4:1–93 8 pls
Hildebrand T, Rüegsegger P (1997) A new method for the model-independent assessment of thickness in three-dimensional images. J Micros Oxford 185:67–75
Kak C, Slaney M (1987) Principles of computerized tomographic imaging. IEEE Press
Lindner A, Cairns SD, Cunningham CW (2008) From offshore to onshore: multiple origins of shallow-water corals from deep-sea ancestors. PLoSONE 3(6):e2429
Miglietta MP (2006) Hydractinia antonii sp. nov.: a new, partially calcified hydractiniid (Cnidaria, Hydrozoa, Hydractiniidae) from Alaska. J Mar Biol Ass UK 86:993–996
Miglietta MP, McNally L, Cunningham CW (2010) Evolution of calcium-carbonate skeletons in the hydractiniidae. Integr Comp Biol 50(3):428–435
Millard N (1975) Monograph on the Hydroida of southern Africa. Ann S Afr Mus 68:1–513
Moseley HN (1879) On the structure of the Stylasteridae, a family of the hydroid stony corals. Phil Trans Roy Soc Lond Ser B 169:425–503
Moseley HN (1881) Report on certain Hydroid, Alcyonarian and Madreporarian corals procured during the voyage of H.M.S. Challenger in the years 1873–1876. Part 1. On the Hydrocorallinae. Rep Sci Res Voyage Chall Zool 2:1–101 209–230, pls 1–14
Ostarello GL (1973) Natural history of the hydrocoral Allopora californica Verrill (1866). Biol Bull Mar Biol Lab Woods Hole 145:548–564
Puce S, Bo M, Di Camillo CG, Paoli L, Pica D, Bavestrello G (2010) Morphology and development of the early growth stages of an Indonesian Stylaster (Cnidaria: Hydrozoa). J Mar Biol Ass UK 90(6):1145–1151
Rosenfeld A, Kak AC (1982) Digital picture processing, 2nd ed. Academic Press, Inc
Schönberg HL, Shields G (2008) Micro-computed tomography for studies on Entobia: transparent substrate versus modern technology. In: Wisshak M, Tapanila L (eds) Current developments in bioerosion. Erlangen Earth Conference Series. Springer-Verlag, Berlin, pp 147–164
Soille P (2004) Morphological image analysis: principles and applications, 2nd edn. Springer, Berlin
Stechow E (1921) Neue Gruppen skelettbildender Hydrozoen und Verwandtschaftsbeziehungen rezenter und fossiler Formen. Verh dt Zool Ges 26:29–31
Wisshak M, Lopez Correa M, Zibrowius H, Jakobsen J, Freiwald A (2009) Skeletal reorganisation affects geochemical signals, exemplified in the stylasterid hydrocoral Errina dabneyi (Azores Archipelago). Mar Ecol Prog Ser 397:197–208
This work was financially supported by Ministero degli Affari Esteri (Grande Rilevanza). The comments provided by Dr. Helmut Zibrowius and an anonymous referee greatly improved the manuscript.
Communicated by T. Bartolomaeus.
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Puce, S., Pica, D., Mancini, L. et al. Three-dimensional analysis of the canal network of an Indonesian Stylaster (Cnidaria, Hydrozoa, Stylasteridae) by means of X-ray computed microtomography. Zoomorphology 130, 85–95 (2011). https://doi.org/10.1007/s00435-011-0120-5
- Canal network
- X-ray microtomography
- 3D analysis