A three-platelet mRNA set: MAX, MTURN and HLA-B as biomarker for lung cancer
During the development of tumors, tumors “educate” platelets causing changes in their mRNAs expression profiles and phenotypes, thereby, tumor-educated platelet (TEP) mRNA profile has the potential to diagnose lung cancer. The current study aimed to examine whether TEPs might be a potential biomarker for lung cancer diagnostics.
Platelet precipitation was obtained by low-speed centrifugation and subjected to Trizol for total RNA extraction. Platelet MAX, MTURN, and HLA-B mRNA were selected by microarray, validated by qPCR, and analyzed combined with related clinical factors.
Our results showed that a three-platelet mRNA set: MAX, MTURN, and HLA-B was significantly up-regulated in lung cancer patients as well as in early-stage lung cancer patients compared with those from healthy donors, the area under the curve (AUC) was 0.734, 0.787, respectively, among which platelet MTURN mRNA processed a dramatically high diagnostic efficiency in female patients with lung cancer, its AUC for female was 0.825. More importantly, the three-platelet mRNA set: MAX, MTURN, and HLA-B was associated with chemotherapeutic effect, low mRNA expression of this three-platelet set was correlated with “favorable” first chemotherapy response.
A three-platelet mRNA set: MAX, MTURN and HLA-B enables blood-based lung cancer diagnosis and chemotherapy response prediction.
KeywordsTEPs Platelet mRNA MAX MTURN HLA-B UQCRH Biomarker
This work was supported by the National Natural Science Foundation of China (81773237, 81672104), the Shandong Provincial Key Research and Development Program (2016GSF201211, 2016GSF201162, 2017GSF18183 and 2017CXGC1207).
Compliance with ethical standards
Conflict of interest
The authors declare that there are no conflicts of interest.
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