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Does rarity mean imparity? Biological characteristics of osteosarcoma cells originating from the spine

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Abstract

Purpose

Osteosarcoma is one of the most common malignancies in bones and is often found in limbs. Until now, it is not clear why osteosarcoma is rare in the spine. On the other hand, previous biological characteristics study about osteosarcoma of spine was also rare because of its low incidence. To explore the biology of spinal osteosarcoma, a stable osteosarcoma cell line derived from spine is necessary.

Methods

A novel osteosarcoma cell line named NEO217 was established from spinal osteosarcoma tissues obtained from a Chinese male patient. We performed a series of experiments to investigate the biological properties of NEO217, including cell morphology, the kinetics of cell growth, biomarkers and tumorigenicity.

Results

The cell line NEO217 was passaged in vitro for more than 50 generations. Ultramicroscopic structural features of these cells were consistent with the pleomorphism characteristic of cancer cells. The average cell doubling time was 26 h. The chromosomal morphology was that of a human karyotype, with the number of chromosomes more than 80. NEO217 cells and available osteosarcoma cell lines such as MG-63 and MNNG/HOS were all CD29+CD59+ phenotype as detected by flow cytometry. Inoculation of NEO217 cells to immunodeficient mice led to tumor formation. The biological and molecular properties of NEO217 cell line are not exactly the same as some human osteosarcoma cell lines derived from the extremities.

Conclusion

We have established a novel osteosarcoma cell line NEO217 derived from the spine, which will provide a useful model for biological or therapeutical studies of spinal osteosarcoma.

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Author information

Correspondence to Jianru Xiao.

Ethics declarations

Conflict of interest

The corresponding author Dr. Jianru Xiao has received research grants from funding listed below in Funding Source. The authors declare that they have no conflict of interest.

Funding source

This study was funded by Bone Tumors and Nervous System Tumors Biobank Project of Shanghai (12DZ2295103), Bone Tumor Sample Databases and Digital Information Platform Project of Shanghai (08DZ2292800), and Shanghai Biobank Network of Common Human Tumor Tissue (12DZ2295100).

Ethical approval

(1) Statement of human rights: All procedures performed in studies involving human participants were in accordance with the ethical standards of the Clinical Research Ethics Committee of Second Military Medical University and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards. Tumor tissues were obtained with informed consent, and this study was approved by the Clinical Research Ethics Committee of the Second Military Medical University. (2) Statement on the welfare of animals: All procedures performed in studies involving animals were in accordance with the ethical standards of the United Kingdom Coordinating Committee on Cancer Prevention Research’s Guidelines for the Welfare of Animals in Experimental Neoplasia. All experimental operations on animals were approved by the Animal Ethics Committees of the Second Military Medical University.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Zhou, Z., Li, Y., Yan, X. et al. Does rarity mean imparity? Biological characteristics of osteosarcoma cells originating from the spine. J Cancer Res Clin Oncol 143, 1959–1969 (2017). https://doi.org/10.1007/s00432-017-2448-9

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Keywords

  • Osteosarcoma
  • Spine
  • Biomarker
  • Cell line
  • Tumor biology