To determine the involvement of ZIC2 in oral squamous cell carcinoma (OSCC).
ZIC2 mRNA and protein expression in primary OSCCs (n = 74), oral premalignant lesions (OPLs, n = 20) and five OSCC-derived cell lines (HSC-2, HSC-3, OK-92, H1, and Sa3) were analyzed by quantitative reverse transcriptase-polymerase chain reaction, Western blot and immunohistochemistry (IHC). In addition, we evaluated the correlation between ZIC2 IHC scores in OSCCs and the clinicopathologic status.
Significant up-regulation of ZIC2 was detected in OSCC-derived cell lines (P < 0.05), primary OSCCs (P < 0.05) and OPLs (P < 0.05) compared with normal counterparts. Among the clinical variables analyzed, ZIC2 expression was associated with the histopathologic types of OSCC. Furthermore, the survival rates differed significantly between ZIC2-positive cases and ZIC2-negative cases.
These results suggested that ZIC2 expression is correlated with the differentiation type of OSCC and diagnosis and might be a potential prognostic indicator and therapeutic target for OSCCs.
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This study was supported by a Grant-in-Aid Scientific Research (No. 20592353) from Ministry of Education, Culture, Sports, Science and Technology of Japan. We thank Lynda C. Charters for editing this manuscript.
Conflict of interest statement
We declare that we have no conflict of interest.
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Sakuma, K., Kasamatsu, A., Yamatoji, M. et al. Expression status of Zic family member 2 as a prognostic marker for oral squamous cell carcinoma. J Cancer Res Clin Oncol 136, 553–559 (2010). https://doi.org/10.1007/s00432-009-0689-y
- Oral squamous cell carcinoma
- Zic family member 2
- Oral premalignant lesion