Journal of Cancer Research and Clinical Oncology

, Volume 135, Issue 11, pp 1625–1633 | Cite as

Characterization of intracellular superoxide dismutase alterations in premalignant and malignant lesions of the oral cavity: correlation with lymph node metastasis

  • Hidetaka Yokoe
  • Hitomi Nomura
  • Yukio Yamano
  • Kazuaki Fushimi
  • Yosuke Sakamoto
  • Katsunori Ogawara
  • Masashi Shiiba
  • Hiroki Bukawa
  • Katsuhiro Uzawa
  • Yuichi Takiguchi
  • Hideki TanzawaEmail author
Original Paper



The purpose of this study was to characterize changes in the expression of copper–zinc superoxide dismutase (Cu/Zn-SOD) and manganese SOD (Mn-SOD) in oral squamous-cell carcinoma (OSCC).


Real-time quantitative reverse transcriptase-polymerase chain reaction analysis of Cu/Zn-SOD and Mn-SOD mRNA expression was carried out in 50 pairs of OSCC tissue specimens and corresponding normal tissues. Mn-SOD protein expression was evaluated further in 65 OSCC tissue samples and 33 oral premalignant lesions (OPLs) using immunohistochemistry.


Significant (P < 0.001) upregulation of Mn-SOD mRNA expression was observed in OSCC tissues compared with the normal tissue counterparts, whereas no significant difference was detected in Cu/Zn-SOD expression. Significant increases in Mn-SOD protein expression were seen in both OPLs (P < 0.001) and OSCC tissue (P < 0.001) together with a high incidence of lymph node metastasis (P = 0.04).


Our findings suggested that Mn-SOD overexpression is a frequent and early event during oral carcinogenesis and could contribute to aggressive OSCC.


Superoxide dismutase Immunohistochemistry Oral squamous-cell carcinoma Oral premalignant lesion Lymph node metastasis 



We thank Lynda C. Charters for editing this manuscript. This study was partly supported by a Grant-in-Aid Scientific Research (No. 20791492) from the Ministry of Education, Culture, Sports, Science and Technology of Japan and Global COE Program (Global Center for Education and Research in Immune System Regulation and Treatment), MEXT, Japan.


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Copyright information

© Springer-Verlag 2009

Authors and Affiliations

  • Hidetaka Yokoe
    • 3
  • Hitomi Nomura
    • 1
  • Yukio Yamano
    • 1
  • Kazuaki Fushimi
    • 1
  • Yosuke Sakamoto
    • 3
  • Katsunori Ogawara
    • 1
  • Masashi Shiiba
    • 1
  • Hiroki Bukawa
    • 3
  • Katsuhiro Uzawa
    • 1
    • 3
  • Yuichi Takiguchi
    • 2
  • Hideki Tanzawa
    • 1
    • 3
    Email author
  1. 1.Department of Clinical Molecular Biology, Graduate School of MedicineChiba UniversityChibaJapan
  2. 2.Department of Respirology, Graduate School of MedicineChiba UniversityChibaJapan
  3. 3.Division of Dentistry and Oral-Maxillofacial SurgeryChiba University HospitalChibaJapan

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