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Human cervical carcinoma xenograft models for studies of the physiological microenvironment of tumors

Abstract

Objective

To establish and characterize experimental tumor models of advanced squamous cell carcinoma of the uterine cervix.

Methods

Permanent cell lines (CK-160 and TS-415) were established from pelvic lymph node metastases of two cervical carcinoma patients. Xenografted tumors were initiated by inoculating 5 × 105 cells into the gastrocnemius muscle of BALB/c nu/nu mice. The tumors were characterized with respect to histological appearance, fraction of necrotic tissue (NF), pimonidazole hypoxic fraction (HFPim), interstitial fluid pressure (IFP), extracellular pH (pHe), metastatic propensity, and radiation sensitivity.

Results

The xenografted tumors reflected the donor patients’ tumors in histological appearance, metastatic propensity, and radiation sensitivity and showed significant intertumor heterogeneity in growth rate, NF, HFPim, IFP, and pHe.

Conclusions

CK-160 and TS-415 xenografts possess properties making them relevant models for studies of the physiological microenvironment of cervical carcinoma and its influence on metastatic dissemination and response to treatment.

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Acknowledgment

This work was supported by the Norwegian Cancer Society.

Author information

Correspondence to Einar K. Rofstad.

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Ellingsen, C., Natvig, I., Gaustad, J. et al. Human cervical carcinoma xenograft models for studies of the physiological microenvironment of tumors. J Cancer Res Clin Oncol 135, 1177–1184 (2009). https://doi.org/10.1007/s00432-009-0558-8

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Keywords

  • Cervical carcinoma
  • Metastasis
  • Radiosensitivity
  • Hypoxia
  • Extracellular pH
  • Interstitial fluid pressure