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Medical Microbiology and Immunology

, Volume 208, Issue 2, pp 253–258 | Cite as

Kinetics of Alphatorquevirus plasma DNAemia at late times after allogeneic hematopoietic stem cell transplantation

  • Eliseo Albert
  • Carlos Solano
  • Estela Giménez
  • Daniele Focosi
  • Ariadna Pérez
  • Lisa Macera
  • José Luis Piñana
  • Eva María Mateo
  • Juan Carlos Hernández Boluda
  • Fabrizio Maggi
  • David NavarroEmail author
Original Investigation
  • 76 Downloads

Abstract

Torque teno virus (TTV) plasma DNA load has been consistently shown to be a surrogate biomarker of immunosuppression in solid organ transplant recipients. It is uncertain whether it may behave similarly in allogeneic hematopoietic stem cell transplant recipients (allo-HSCT). Here, we characterized the dynamics of TTV DNAemia in patients undergoing T-cell replete allo-SCT at late times after transplantation (> day + 100). This retrospective single-center observational study included 33 allo-HSCT patients. Plasma TTV DNA loads were quantified by real-time PCR before initiating the conditioning regimen and at different time points after transplant. Absolute lymphocyte counts (ALC) were measured by flow cytometry. Overall, TTV DNA load increased steadily after engraftment, reaching a peak by day + 90; afterwards, it remained relatively constant until day + 210. TTV DNA loads measured within days + 120 and + 210 correlated inversely with paired ALC, while both parameters did correlate directly within days + 20 and + 60. The median TTV DNA area under a curve between days + 90 and + 210 [(AUC)90–210] was significantly higher in patients who received corticosteroids within this time frame for treatment of graft versus host disease (either acute, chronic or both) than in controls (P = 0.025). In summary, TTV DNA load may mirror the degree of immunosuppression at late times after allo-HSCT.

Keywords

Torque teno virus (TTV) DNAemia Allogeneic hematopoietic stem cell transplantation Immunosuppression Biomarker 

Notes

Acknowledgements

We would like to thank Juan Manuel Moya Serrano for his technical assistance. Estela Giménez holds a Río-Hortega Contract from the Carlos III Health Institute (ISCIII) (Ref. CM16/00200). Eva María Mateo is grateful to Ministry of Economy and Competitiveness (MINECO, Spanish Government) for a postdoctoral contract “Juan de la Cierva” (Ref. FJCI-2015-25992).

Funding

None.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

430_2019_586_MOESM1_ESM.doc (50 kb)
Supplementary material 1 (DOC 49 KB)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Eliseo Albert
    • 1
  • Carlos Solano
    • 2
    • 3
  • Estela Giménez
    • 1
  • Daniele Focosi
    • 4
  • Ariadna Pérez
    • 2
  • Lisa Macera
    • 5
  • José Luis Piñana
    • 2
  • Eva María Mateo
    • 1
  • Juan Carlos Hernández Boluda
    • 2
  • Fabrizio Maggi
    • 5
    • 6
  • David Navarro
    • 1
    • 7
    Email author
  1. 1.Microbiology Service, Hospital Clínico UniversitarioInstitute for Research INCLIVAValenciaSpain
  2. 2.Hematology Service, Hospital Clínico UniversitarioInstitute for Research INCLIVAValenciaSpain
  3. 3.Department of Medicine, School of MedicineUniversity of ValenciaValenciaSpain
  4. 4.North-Western Tuscany Blood BankPisa University HospitalPisaItaly
  5. 5.Virology UnitPisa University HospitalPisaItaly
  6. 6.Department of Translational ResearchUniversity of PisaPisaItaly
  7. 7.Department of Microbiology, School of MedicineUniversity of ValenciaValenciaSpain

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