Constitutional abnormality of nuclear membrane proteins in small cell lung carcinoma

  • Jieying WangEmail author
  • Tetsuo Kondo
  • Tadao Nakazawa
  • Naoki Oishi
  • Kunio Mochizuki
  • Ryohei Katoh
Original Article


Nuclear membrane proteins reportedly play important roles in maintaining nuclear structures and coordinating cell activities. Studying profiles of nuclear membrane proteins may help us evaluate the biological and/or clinical nature of malignant tumors. Using immunohistochemistry with antibodies for emerin, lamin A/C, lamin B, and LAP2, we examined 105 lung cancer tissues from 33 small cell lung carcinomas (SCLCs) and 72 non-SCLCs (34 adenocarcinomas, 30 squamous cell carcinomas, and 8 large cell carcinomas). Emerin had negative or local/weak positivity in 79% of SCLCs and 1% of non-SCLCs, and lamin A/C had similar positivity in 91% of SCLCs and 3% of non-SCLCs. LAP2’s expression was similar between SCLCs and non-SCLCs. RT-PCR analyses for these four nuclear membrane proteins over 7 cell lines showed that mRNA of emerin and lamin A/C were distinctly downregulated in the SCLC cell lines, supporting the immunohistochemical results. In conclusion, we suggest that downregulation of the nuclear membrane proteins emerin and lamin A/C is characteristic of SCLC cells, and this constitutional abnormality of the nuclear membrane may be related to the biological and/or clinical nature of SCLC. In addition, knowing the nuclear protein profile in SCLC cells may contribute to our understanding of nuclear fragility known as the crush artifact in pulmonary biopsy specimens.


Nuclear membrane proteins Lung carcinoma Immunohistochemistry Polymerase chain reaction (PCR) 



The authors thank Ms. Mikiko Yoda, Ms. Miyuki Ito, Mr. Yoshihito Koshimizu, and Ms. Wakaba Iha for technical support and Ms. Kayoko Kono for executive assistance.


Wang Jieying, Tetsuo Kondo, and Ryohei Katoh conceived and designed the study, and wrote, edited, and reviewed the manuscript. Wang Jieying and Tetsuo Kondo performed the experiment. Tadao Nakazawa, Naoki Oishi, and Kunio Mochizukia researched and analyzed data. All authors gave final approval for publication.


This study was funded by the Natural Science Foundation of Shaanxi Provincial Department of Education (grant number 18JK0666).

Compliance with ethical standards

The study protocols were approved by the Institutional Ethics Board of the University of Yamanashi, Yamanashi, Japan. The study complies with all ethical standards as stated in the Ethical Responsibilities of Authors on the Virchows Archiv webpage (

Conflict of interest

The authors declare that they have no conflicts of interest.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Pathology, Interdisciplinary Graduate School of Medicine and EngineeringUniversity of YamanashiYamanashiJapan
  2. 2.Department of Public HealthXi’an Medical UniversityXi’anChina

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