Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

In pulmonary sclerosing hemangioma expression of β-catenin, Axin, and C-myc differs between the two cell types

Abstract

Previous studies have shown that pulmonary sclerosing hemangioma is a tumor derived from primitive respiratory epithelium, but its character and the differentiation status of the two cell types (polygonal and cuboidal) composing the lesion are still controversial. We hypothesize that the polygonal cells are immature compared with cuboidal cells and have higher proliferative activity. To further study this question, we examined the expression of β-catenin, Axin, and C-myc by immunostaining in 45 primary sclerosing hemangioma (PSH) specimens. The two cell types were captured by laser capture microdissection from 28 PSH specimens, and total RNA was extracted. Messenger RNA (mRNA) expression of Axin and C-myc was examined by reverse transcription polymerase chain reaction (RT-PCR). By immunostaining, β-catenin was predominantly strongly expressed on the cell membrane of cuboidal cells, while in polygonal cells, β-catenin was predominantly expressed in the cytoplasm and significantly decreased on cell membranes. Axin was expressed in cuboidal cells in 93 % of our 45 cases, but only expressed in 18 % of these in polygonal cells. C-myc expression in polygonal cells was significantly stronger than in cuboidal cells (P < 0.05). RT-PCR showed that the expression level of Axin mRNA in cuboidal cells was significantly higher than in polygonal cells (P < 0.05), and expression level of C-myc mRNA in polygonal cells was significantly higher than in cuboidal cells (P < 0.05).The two PHS cell types have distinct expression of β-catenin, Axin, and C-myc, suggesting that their differentiation status may be different. The higher expression of C-myc in polygonal cells suggests that these cells might have higher proliferative activity.

This is a preview of subscription content, log in to check access.

Fig. 1
Fig. 2
Fig. 3
Fig. 4

References

  1. 1.

    Liebow A, Hubbell DS (1956) Sclerosing hemangioma (histiocytoma, Xanthoma) of the lung. Cancer 9(1):53–75

  2. 2.

    Chan AC, Chan JK (2001) Pulmonary sclerosing hemangioma consistently expresses thyroid transcription facter-1: a new clue to its histogenesis. Arch Pathol Lab Med 125(10):1335–1339

  3. 3.

    Wang E, Lin D, Wang Y et al (2004) Immunohistochemical and ultrastructural markers suggest different origins for cuboidal and polygonal cells in pulmonary sclerosing hemangioma. Hum Pathol 35(4):503–508

  4. 4.

    Xu HM, Li WH, Hou N et al (1997) Neuroendocrine differentiation in 32 cases of so-called sclerosing hemangioma of the lung: identified by immunohistochemical and ultrastructural study. Am J Surg Pathol 21(9):1013–1022

  5. 5.

    Hill GS, Eggleston JC (1972) Electron microscopic study of so-called "pulmonary sclerosing hemangioma". Report of a case suggesting epithelial origin. Cancer 30(4):1092–1096

  6. 6.

    Kay S, Still WJ, Borochovitz D (1977) Sclerosing hemangioma of the lung: an endothelial or epithelial neoplasm? Hum Pathol 8(4):468–474

  7. 7.

    Katzenstein AL, Weise DL, Fulling K, Battifora H (1983) Neuroendocrine differentiation in 32 cases of so-called sclerosing hemangioma of the lung: identified by immunohistochemical and ultrastructural study. Am J Surg Pathol 7(1):3–14

  8. 8.

    Niho S, Suzuki K, Yokose T et al (1998) Monoclonality of both pale cells and cuboidal cells of sclerosing hemangioma of the lung. Am J Pathol 152(4):1065–1069

  9. 9.

    Wang EH, Dai SD, Qi FJ et al (2007) Gene expression and clonality analysis of the androgen receptor and phosphoglycerate kinase genes in polygonal cells and cuboidal cells in so-called pulmonary sclerosing hemangioma. Mod Pathol 20(11):1208–1215

  10. 10.

    Nagata N, Dairaku M, Sueishi K, Tanaka K et al (1987) Sclerosing hemangioma of the lung. An epithelial tumor composed of immunohistochemically heterogenous cells. Am J Clin Pathol 88(5):552–559

  11. 11.

    Devouassoux-Shisheboran M, Hayashi T, Linnoila RI et al (2000) A clinicopathologic study of 100 cases of pulmonary sclerosing hemangioma with immunohistochemical studies: TTF-1 is expressed in both round and surface cells, suggesting an origin from primitive respiratory epithelium. Am J Surg Pathol 24(7):906–916

  12. 12.

    Yamazaki K (2004) Type-II pneumocyte differentiation in pulmonary sclerosing hemangioma: ultrastructural differentiation and immunohistochemical distribution of lineage-specific transcription factors (TTF-1, HNF-3 alpha, and HNF-3 beta) and surfactant proteins. Virchows Arch 445:45–53

  13. 13.

    Miyagawa-Hayashino A, Tazelaar HD, Langel DJ, Colby TV (2003) Pulmonary sclerosing hemangioma with lymph node metastases: report of 4 cases. Arch Pathol Lab Med 127:321–325

  14. 14.

    Tanaka I, Inoue M, Matsui Y et al (1986) A case of pneumocytoma (so-called sclerosing hemangioma) with lymph node metastasis. Jpn J Clin Oncol 16:77–86

  15. 15.

    Suzuki H, Saitoh Y, Koh E et al (2011) Pulmonary sclerosing hemangioma with pleural dissemination: report of a case. Surg Today 41(2):258–261

  16. 16.

    Bae YS, Ro JY, Shim HS et al (2012) Pulmonary sclerosing haemangioma with metastatic spread to stomach. Histopathology. doi:10.1111/j.1365-2559.2012.04213.x

  17. 17.

    Mazieres J, He B, You L et al (2005) Wnt signaling in lung cancer. Cancer Lett 222(1):110

  18. 18.

    Hong-Tao Xu, Wang L, Wang En-Hua et al (2006) Abnormal β-catenin and Reduced Axin Expression Are Associated With Poor Differentiation and Progression in Non–Small Cell Lung Cancer. Am J Clin Pathol 125:534–541

  19. 19.

    Jaiswal AS, Kennedy CH, Narayan S (1999) A correlation of APC and c-myc mRNA levels in lung cancer cell lines. Oncol Rep 6(6):1253–1256

  20. 20.

    Dacic S, Sasatomi E, Swalsky PA et al (2004) Loss of heterozygosity patterns of sclerosing hemangioma of the lung and bronchioloalveolarcarcinoma indicate a similar molecular pathogenesis. Arch Pathol Lab Med 128(8):880–884

  21. 21.

    Wang Y, Dai SD, Qi FJ et al (2008) p53 protein expression and genetic mutation in two primary cell types in pulmonary sclerosing haemangioma. J Clin Pathol 61(2):192–196

  22. 22.

    Hosaka N, Sasaki T, Adatoshi K et al (2004) Pulmonary sclerosing hemangioma associated with familial adenomatous polyposis. Hum Pathol 35(6):764–768

  23. 23.

    Kayser K, Trott J, Böhm G et al (2005) Localized fibrous tumors (LFTs) of the pleura: clinical data, asbestos burden, and syntactic structure analysis applied to newly defined angiogenic/growth-regulatory effectors. Pathol Res Pract 201(12):791–801

  24. 24.

    Anani W, Bruggeman R, Zander DS (2011) β-catenin expression in benign and malignant pleural disorders. Int J Clin Exp Pathol 4(8):742–747

  25. 25.

    Andino L, Cagle PT, Murer B et al (2006) Pleuropulmonary desmoid tumors immunohistochemical comparison with solitary fibrous tumors and assessment of β-Catenin and cyclin D1 expression. Arch Pathol Lab Med 130:1503–1509

  26. 26.

    Yang LH, Xu HT, Han Y et al (2010) Axin downregulates TCF-4 transcription via beta-catenin, but not p53, and inhibits the proliferation and invasion of lung cancer cells. Mol Cancer 2(9):25

  27. 27.

    Chiba W, Sawai S, Yasuda Y et al (1995) Positive expression of c-myc and p53 products in two cases of pulmonary sclerosing hemangioma. Nihon Kyobu Shikkan Gakkai Zasshi 33(12):1348–1354

  28. 28.

    Liu W, Tian X-Y, Li Y, et al (2011) Coexistence of pulmonary sclerosing hemangioma and primary adenocarcinoma in the same nodule of lung. Diagn Pathol 6:41

Download references

Acknowledgments

All lung tissue samples were obtained from The First Affiliated Hospital of China Medical University. The study was conducted according to the regulations of the institutional review boards at China Medical University

Conflict of interest

The authors declare that they have no competing financial interests. No part of this article has been published or submitted elsewhere, and there are no financial or other relationships that might lead to a conflict of interest of this article. The manuscript has been read and approved by all the authors, that the requirements for authorship have been met, and that each author believes that the manuscript represents honest work.

Author information

Correspondence to En-Hua Wang.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Lin, X., Zhang, D., Zhang, Y. et al. In pulmonary sclerosing hemangioma expression of β-catenin, Axin, and C-myc differs between the two cell types. Virchows Arch 461, 59–65 (2012). https://doi.org/10.1007/s00428-012-1247-6

Download citation

Keywords

  • Pulmonary sclerosing hemangioma
  • Laser capture microdissection (LCM)
  • Wnt signaling pathway