Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Retinoic acid disrupts anterior ectodermal and endodermal development in ascidian larvae and postlarvae

Abstract

 In vertebrates, excess all-trans retinoic acid (RA) applied during axis formation leads to the apparent truncation of anterior structures. In this study we sought to determine the type of defects caused by ectopic RA on the development of the ascidian Herdmania curvata. We demonstrate that H. curvata embryos cultured in the presence of RA develop into larvae whose trunks are shortened and superficially resemble those of early metamorphosing postlarvae. Despite RA-treated larvae lacking papillar structures they respond normally to natural cues that induce metamorphosis, indicating that chemosensory functionality previously mapped to the most anterior region of normal larvae is unaffected by RA. Excess RA applied during postlarval development leads to a graded loss of the juvenile pharynx, apparently by respecifying anterior endoderm to a more posterior fate. This structure is considered homologous to the gill slits of amphioxus, which are also lost upon RA treatment. This suggests that RA may have had a role in the development of the pharynx of the ancestral chordate and that this function has been maintained in ascidians and cephalochordates and lost in vertebrates.

This is a preview of subscription content, log in to check access.

Author information

Additional information

Received: 27 February 1998 / Accepted: 20 April 1998

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Hinman, V., Degnan, B. Retinoic acid disrupts anterior ectodermal and endodermal development in ascidian larvae and postlarvae. Dev Gene Evol 208, 336–345 (1998). https://doi.org/10.1007/s004270050189

Download citation

  • Key words Retinoic acid
  • Ascidian
  • Anteroposterior Patterning
  • Herdmania curvata