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Development Genes and Evolution

, Volume 207, Issue 2, pp 90–96 | Cite as

Inductive interactions required for mesodermal differentiation in Bufo arenarum gastrulae

  • M. E. Manes
  • Fernando Campos Casal
ORIGINAL ARTICLE

Abstract

 Dorso-marginal epithelium, a distinct crescent-shaped region in the early gastrula of Bufo arenarum, appears after involution as a narrow dorso-median strip of archenteric endoderm close to the notochord. In explant cultures, this layer showed an extreme dorsalizing activity, promoting the formation of notochordal structures from ventro-mesodermal cells. In the presence of ectoderm, this inductive activity was expanded resulting in a wide range of dorso-lateral components such as notochord, muscle, nephric tubules, mesothelium and mesenchyme. The mesodermal origin of these derivatives was confirmed by the use of FDA (fluorescein-dextran-amine)-labelled explants. Extensive mesodermal development in cultures seems to require cell contacts between the inner aspect of the dorso-marginal epithelium and mesodermal cells. When such contacts were prevented, cultures would only differentiate erythrocytes as a result of a purely ectodermal stimulus. Bisection of the dorso-marginal epithelium in whole embryos resulted in the development of a duplicated set of axial structures, clearly showing the role of the epithelium as a dorsal organizer.

Key words amphibian morphogenesis organizer mesoderm dorsalization differentiation 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1997

Authors and Affiliations

  • M. E. Manes
    • 1
  • Fernando Campos Casal
    • 1
  1. 1.Cátedra de Histología y Embriología, Facultad de Agronomía y Zootecnia, Universidad Nacional de Tucumán, 4000, Tucumán, ArgentinaAR

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