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Development Genes and Evolution

, Volume 225, Issue 2, pp 105–111 | Cite as

Developmental expression analysis of Na, K-ATPase α subunits in Xenopus

  • Md. Mahfujur Rahman
  • Hyun-Jin Tae
  • Ho-Seong Cho
  • Gee-Wook Shin
  • Byung-Yong ParkEmail author
Sequence Corner

Abstract

Na, K-ATPase is an integral membrane protein complex responsible for maintaining the ionic gradients of Na+ and K+ across the plasma membrane and has a variety of cellular functions including neuronal activity. Studies in several organisms have shown that this protein complex regulates multiple aspects of embryonic development and is responsible for the pathogenesis of several human diseases. Here, we report the cloning and expression of Na, K-ATPase α2 (atp1a2) and α3 (atp1a3) subunits during Xenopus development and compare the expression patterns of each subunit. Using in situ hybridization in whole embryos and on sections, we show that all three α subunits are co-expressed in the pronephric kidney, with varying expression in neurogenic derivatives. The atp1a2 has a unique expression in the ependymal cell layer of the developing brain that is not shared with other α subunits. The Na, K-ATPase α1 (atp1a1), and atp1a3 share many expression domains in placode derivatives, including the otic vesicle, lens, ganglion of the anterodorsal lateral line nerve, and ganglia of the facial and anteroventral lateral line nerve and olfactory cells. All the subunits share a common expression domain, the myocardium.

Keywords

Na K-ATPase α subunits Brain Pronephric kidney Eye Placode derivatives Xenopus 

Notes

Acknowledgments

This study was financially supported by grants from the Korea Research Foundation Grant (2011–0014454).

Compliance with ethical standard

Conflict of interest

The authors declare that they have no conflict of interest.

Statement of the welfare of animals

All procedures performed in studies involving animals were in accordance with the ethical standards of the animal welfare regulations of Institutional Animal Care and Use Committees (IACUC), Chonbuk National University Laboratory Animal Centre, Republic of Korea.

Informed consent

Informed consent was obtained from all individual participants included in the study.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2015

Authors and Affiliations

  • Md. Mahfujur Rahman
    • 1
  • Hyun-Jin Tae
    • 2
  • Ho-Seong Cho
    • 1
  • Gee-Wook Shin
    • 1
  • Byung-Yong Park
    • 1
    • 3
    Email author
  1. 1.Bio-Safety Research Institute, College of Veterinary MedicineChonbuk National UniversityJeonjuRepublic of Korea
  2. 2.Institute of Bioscience and BiotechnologyHallym UniversityChuncheonRepublic of Korea
  3. 3.Department of Veterinary Embryology, College of Veterinary MedicineChonbuk National UniversityJeonjuRepublic of Korea

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