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Possible implication of Hox genes Abdominal-B and abdominal-A in the specification of genital and abdominal segments in cirripedes

Abstract

The crustaceans cirripedes (barnacles) are characterised by the lack of fully developed abdominal segments at any stage of their life cycle. However, in nauplius larvae of the cirripede Sacculina carcini, we detected five small engrailed stripes in a postero-dorsal region behind the sixth thoracic segment, that we interpreted as a vestigial abdomen. Here, we present additional morphological and genetic data on Sacculina to further characterise this structure. Scanning electron microscopy analysis confirms the existence of a segmented region in this part of the naupliar body. However, at the late naupliar stage, this structure stops its development and degenerates. This region expresses the Hox gene Abdominal-B, which may indicate that it actually corresponds to the posterior-most part of the Sacculina trunk. In addition, Abdominal-B expression differentiates two types of larvae that probably correspond to male and female larvae, respectively. In contrast, no abdominal-A expression can be detected in the vestigial abdomen. We discuss the possible implication of the loss or divergence of the Abdominal-A protein in the impaired development of abdominal segments in cirripedes.

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References

  1. Abzhanov A, Kaufman TC (2000) Embryonic expression patterns of the Hox genes of the crayfish Procambarus clarkii (Crustacea, Decapoda). Evol Dev 2:271–283

  2. Anderson DT (1994) Barnacles: structure, function, development and evolution. Chapman and Hall, London

  3. Averof M, Akam M (1995) Hox genes and the diversification of insect and crustacean body plans. Nature 376:420–423

  4. Boulet AM, Lloyd A, Sakonju S (1991) Molecular definition of the morphogenetic and regulatory functions and the cis-regulatory elements of the Drosophila Abd-B homeotic gene. Development 111:393–405

  5. Casanova J, Sanchez-Herrero E, Morata G (1986) Identification and characterization of a parasegment specific regulatory element of the abdominal-B gene of Drosophila. Cell 47:627–636

  6. Damen WG, Tautz D (1999) Abdominal-B expression in a spider suggests a general role for Abdominal-B in specifying the genital structure. J Exp Zool 285:85–91

  7. Gibert JM, Mouchel-Vielh E, Queinnec E, Deutsch JS (2000) Barnacle duplicate engrailed genes: divergent expression patterns and evidence for a vestigial abdomen. Evol Dev 2:194–202

  8. Glenner H, Hoeg JT (1995) A new motile, multicellular stage involved in host invasion by parasitic barnacles (Rhizocephala). Nature 377:147–150

  9. Kelsh R, Weinzierl RO, White RA, Akam M (1994) Homeotic gene expression in the locust Schistocerca: an antibody that detects conserved epitopes in Ultrabithorax and abdominal-A proteins. Dev Genet 15:19–31

  10. Kolbasov GA, Hoeg JT, Elfimov AS (1999) Scanning electron microscopy of an acrothoracican cypris larvae (Crustacea, Thecostraca, Cirripedia, Acrothoracica, Lithoglyptidae). Contrib Zool 68:143–160

  11. Mouchel-Vielh E, Rigolot C, Gibert JM, Deutsch JS (1998) Molecules and the body plan: the Hox genes of Cirripedes (Crustacea). Mol Phylogenet Evol 9:382–389

  12. Mouchel-Vielh E, Blin M, Rigolot C, Deutsch JS (2002) Expression of a fushi-tarazu homologue in a cirripede crustacean. Evol Dev 4:76–85

  13. Mourlan A, Turquier Y, Baucher MF (1985) Recherche sur l'ontogenèse des rhizocéphales. II. Organisation anatomique de la cypris libre de Sacculina carcini (Thompson). Cah Biol Mar 26:281–300

  14. Rabet N, Gibert JM, Queinnec E, Deutsch JS, Mouchel-Vielh E (2001) The caudal gene of the barnacle Sacculina carcini is not expressed in its vestigial abdomen. Dev Genes Evol 211:172–178

  15. Tear G, Akam M, Martinez-Arias A (1990) Isolation of an abdominal-A gene from the locust Schistocerca gregaria and its expression during early embryogenesis. Development 110:915–925

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Acknowledgements

We thank Dr Rob White for the gift of the FP6.87 antibody, Professor André Toulmond for providing the facilities of the Station de Biologie Marine at Roscoff (Brittany, France) and Professor Habib Bouleckbache for its support.

Author information

Correspondence to Emmanuèle Mouchel-Vielh.

Additional information

Edited by P. Simpson

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Blin, M., Rabet, N., Deutsch, J.S. et al. Possible implication of Hox genes Abdominal-B and abdominal-A in the specification of genital and abdominal segments in cirripedes. Dev Genes Evol 213, 90–96 (2003). https://doi.org/10.1007/s00427-003-0294-z

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Keywords

  • Hox
  • Abdominal-B
  • abdominal-A
  • Cirripede
  • Abdomen