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GFP expression in Hydra: lessons from the particle gun

Abstract.

The cnidarian Hydra is an important model organism to study pattern formation and stem cell differentiation. In the past, however, it has been difficult to study gene function in Hydra because the animals have not been accessible to gene transfection studies. We have now developed a method to transiently express GFP-tagged proteins in Hydra using a green fluorescent protein (GFP) expression plasmid under the control of the Hydra actin promoter and a particle gun to introduce it into Hydra cell nuclei. We achieve strong transient GFP expression in a small but reproducible number of epithelial and interstitial cells. Implications for the use of this method to carry out single cell assays with GFP-tagged Hydra proteins are discussed.

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Böttger, A., Alexandrova, O., Cikala, M. et al. GFP expression in Hydra: lessons from the particle gun. Dev Genes Evol 212, 302–305 (2002). https://doi.org/10.1007/s00427-002-0245-0

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  • GFP Hydra Particle gun