Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Microinjection reveals cell-to-cell movement of green fluorescent protein in cells of maize coleoptiles

  • 106 Accesses

  • 14 Citations

Abstract.

During the evaluation of dual-purpose plant/fungal expression systems, we found that green fluorescent protein (GFP) has the ability to move from cell to cell in the epidermis of Zea mays L. cv. Mutator coleoptiles as well as into underlying cortical cells. Movement of GFP was observed both when DNA encoding GFP and bacterially expressed GFP were microinjected into epidermal cells. This suggests that GFP is capable of cell-to-cell movement. From experiments using dextrans of known molecular weight linked to fluorescein isothiocyanate and tetramethylrhodamine isothiocyanate, we estimate that the plasmodesmata of these cells have a size exclusion limit <4.4 kDa. Cell-to-cell GFP movement did not occur when GFP was altered to include a nucleus- or endoplasmic reticulum-retention sequence. The fact that these transcripts differ from that of cytoplasmic GFP by a small number of nucleotides suggests that the transcripts are not capable of movement, but movement of nucleic acid cannot be excluded. Since GFP is widely used to study cell-to-cell movement and to localize the expression of transgenes, caution should be exercised when interpreting results where GFP expression is used for localization.

This is a preview of subscription content, log in to check access.

Author information

Additional information

Received: 12 June 2000 / Accepted: 26 July 2000

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Wymer, C., Fernández-Ábalos, J. & Doonan, J. Microinjection reveals cell-to-cell movement of green fluorescent protein in cells of maize coleoptiles. Planta 212, 692–695 (2001). https://doi.org/10.1007/s004250000454

Download citation

  • Key words: Cell-to-cell movement
  • Green fluorescent protein
  • Microinjection
  • Plasmodesmata
  • Zea