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Planta

, Volume 250, Issue 6, pp 1941–1953 | Cite as

Rosette core fungal resistance in Arabidopsis thaliana

  • Yanwan Dai
  • Huw A. Ogilvie
  • Yuan Liu
  • Michael Huang
  • Lye Meng Markillie
  • Hugh D. Mitchell
  • Eli J. Borrego
  • Michael V. Kolomiets
  • Matthew J. Gaffrey
  • Galya Orr
  • E. Wassim Chehab
  • Wan-Ting Mao
  • Janet BraamEmail author
Original Article

Abstract

Main conclusion

Unlike rosette leaves, the mature Arabidopsis rosette core can display full resistance to Botrytis cinerea revealing the importance for spatial and developmental aspects of plant fungal resistance.

Abstract

Arabidopsis thaliana is a model host to investigate plant defense against fungi. However, many of the reports investigating Arabidopsis fungal defense against the necrotrophic fungus, Botrytis cinerea, utilize rosette leaves as host tissue. Here we report organ-dependent differences in B. cinerea resistance of Arabidopsis. Although wild-type Arabidopsis rosette leaves mount a jasmonate-dependent defense that slows fungal growth, this defense is incapable of resisting fungal devastation. In contrast, as the fungus spreads through infected leaf petioles towards the plant center, or rosette core, there is a jasmonate- and age-dependent fungal penetration blockage into the rosette core. We report evidence for induced and preformed resistance in the rosette core, as direct rosette core inoculation can also result in resistance, but at a lower penetrance relative to infections that approach the core from infected leaf petioles. The Arabidopsis rosette core displays a distinct transcriptome relative to other plant organs, and BLADE ON PETIOLE (BOP) transcripts are abundant in the rosette core. The BOP genes, with known roles in abscission zone formation, are required for full Arabidopsis rosette core B. cinerea resistance, suggesting a possible role for BOP-dependent modifications that may help to restrict fungal susceptibility of the rosette core. Finally, we demonstrate that cabbage and cauliflower, common Brassicaceae crops, also display leaf susceptibility and rosette core resistance to B. cinerea that can involve leaf abscission. Thus, spatial and developmental aspects of plant host resistance play critical roles in resistance to necrotrophic fungal pathogens and are important to our understanding of plant defense mechanisms.

Keywords

Biotic stress Botrytis cinerea Jasmonate Plant defense RNA-seq 

Abbreviations

BOP

BLADE ON PETIOLE

MeJA

Methyl jasmonate

JA-Ile

Jasmonoyl-l-isoleucine

Notes

Acknowledgements

This work was supported by Rice University, China Scholarship Council (No. 201406350018 to YD), and the E&M Foundation to W-TM. Part of the research was performed using the Environmental Molecular Sciences Laboratory (EMSL), a national scientific user facility sponsored by the Department of Energy’s Office of Biological and Environmental Research and located at Pacific Northwest National Laboratory.

Supplementary material

425_2019_3273_MOESM1_ESM.pdf (1.6 mb)
Supplementary material 1 (PDF 1607 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.BioSciences DepartmentRice UniversityHoustonUSA
  2. 2.Computer Science DepartmentRice UniversityHoustonUSA
  3. 3.Earth and Biological Sciences DirectoratePacific Northwest National LaboratoryRichlandUSA
  4. 4.Department of Plant Pathology and MicrobiologyTexas A&M UniversityCollege StationUSA
  5. 5.Graduate Institute of BiotechnologyNational Chung Hsing UniversityTaichungTaiwan
  6. 6.Thomas H. Gosnell School of Life SciencesRochester Institute of TechnologyRochesterUSA

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