Identification of the phytosulfokine receptor 1 (OsPSKR1) confers resistance to bacterial leaf streak in rice
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A rice allele of PSKR1 functioning in resistance to bacterial leaf streak was identified.
Phytosulfokine (PSK), a disulfated pentapeptide encoded by precursor genes that are ubiquitously present in higher plants, belongs to the group of plant peptide growth factors. The PSK receptor PSKR1 in Arabidopsis thaliana is an active kinase and has guanylate cyclase activity resulting in dual-signaling outputs. Here, the LOC_Os02g41890 out of three candidates completely rescued root growth and susceptible to Pseudomonas syringae pv. DC3000 in the Arabidopsis pskr1-3 mutant and was identified as OsPSKR1. This protein was localized to plasma membrane similar to AtPSKR1. The expression of OsPSKR1 was upregulated upon inoculation with RS105, a strain of Xanthomonas oryzae pv. oryzicola (Xoc) that cause bacterial leaf streak in rice. OsPSKR1 overexpression (OE) lines had greater resistance to RS105 than the wild type. Consistently, the expression of pathogenesis-related genes involved in the salicylic acid (SA) pathway was upregulated in the transgenic lines. Overall, OsPSKR1 functions as a candidate PSK receptor and regulates resistance to Xoc by activating the expression of pathogenesis-related genes involved in the SA pathway in rice.
KeywordsBacterial leaf streak Defense-related gene Phytohormones Phytosulfokine Rice
Bacterial leaf streak
Green fluorescent protein
Xanthomonas oryzae pv. oryzicola
This work is supported by the National Natural Science Foundation of China (31872925, 31700219), the Natural Science Fund for Outstanding Young Scholars of Shandong Province (JQ201807), the Shandong Modern Agricultural Technology & Industry system (SDAIT-17-06).
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
The authors declare that the experiments comply with the laws of China.
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