Phytochrome-interacting factor-like protein OsPIL15 integrates light and gravitropism to regulate tiller angle in rice
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Rice phytochrome-interacting factor-like protein OsPIL15 regulates tiller angle through light and gravity signals in rice.
Tiller angle of cereal crops is a key agronomic trait that contributes to grain production. An understanding of how tiller angle is controlled is helpful for achieving ideal plant architecture to improve grain yield. Phytochrome-interacting factors (PIFs) are known to regulate seed germination, seedling skotomorphogenesis, shade avoidance, and flowering in Arabidopsis thaliana. Here, we report that OsPIL15 is, indeed, a rice PIF that negatively regulates tiller angle. Dominant-negative OsPIL15 plants displayed a larger tiller angle, which was associated with reduced shoot gravitropism. Phytochrome B (phyB) is the main photoreceptor perceiving the low red:far-red ratio of shade light. Compared with wild-type rice plants, loss-of-function phyB plants and OsPIL15-overexpressing plants showed smaller tiller angles and enhanced shoot gravitropism. In addition, more OsPIL15 protein accumulated in phyB plants than in wild-type plants. Light regulates the level of the OsPIL15 protein negatively, depending on phyB partially. We propose that OsPIL15 integrates light and gravity signals to regulate tiller angle in rice.
KeywordsOsPIL15 Tiller angle Phytochrome Gravitropism
This work was supported by the National Transgenic Science and Technology Program (2016ZX08010-002) and the National Basic Research Program (973 Program 2013CBA01403). We are grateful to Dr. Jiayang Li for the vector pVKmp-lib and Dr. Jianmin Wan for rice phyB seeds.
- Cordeiro AM, Figueiredo DD, Tepperman J, Borba AR, Lourenco T, Abreu IA, Ouwerkerk PB, Quail PH, Margarida Oliveira M, Saibo NJ (2016) Rice phytochrome-interacting factor protein OsPIF14 represses OsDREB1B gene expression through an extended N-box and interacts preferentially with the active form of phytochrome B. Biochim Biophys Acta 1859:393–404CrossRefGoogle Scholar
- He Y, Li Y, Cui L, Xie L, Zheng C, Zhou G, Zhou J, Xie X (2016) Phytochrome B negatively affects cold tolerance by regulating OsDREB1 gene expression through phytochrome interacting factor-like protein OsPIL16 in Rice. Front Plant Sci 7:1963Google Scholar
- Hornitschek P, Kohnen MV, Lorrain S, Rougemont J, Ljung K, López-Vidriero I, Franco-Zorrilla JM, Solano R, Trevisan M, Pradervand S, Xenarios I, Fankhauser C (2012) Phytochrome interacting factors 4 and 5 control seedling growth in changing light conditions by directly controlling auxin signaling. Plant J 71:699–711CrossRefGoogle Scholar
- Tanabe S, Ashikari M, Fujioka S, Takatsuto S, Yoshida S, Yano M, Yoshimura A, Kitano H, Matsuoka M, Fujisawa Y, Kato H, Iwasaki Y (2005) A novel cytochrome P450 is implicated in brassinosteroid biosynthesis via the characterization of a rice dwarf mutant, dwarf11, with reduced seed length. Plant Cell 17:776–790CrossRefGoogle Scholar
- Todaka D, Nakashima K, Maruyama K, Kidokoro S, Osakabe Y, Ito Y, Matsukura S, Fujita Y, Yoshiwara K, Ohme-Takagi M, Kojima M, Sakakibara H, Shinozaki K, Yamaguchi-Shinozaki K (2012) Rice phytochrome-interacting factor-like protein OsPIL1 functions as a key regulator of internode elongation and induces a morphological response to drought stress. Proc Natl Acad Sci USA 109:15947–15952CrossRefGoogle Scholar