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Indole-3-acetylaspartate and indole-3-acetylglutamate, the IAA-amide conjugates in the diploid strawberry achene, are hydrolyzed in growing seedlings

  • Qian Tang
  • Peng Yu
  • Molly Tillmann
  • Jerry D. Cohen
  • Janet P. Slovin
Original Article
  • 72 Downloads

Abstract

Main conclusion

Indole-3-acetylaspartate and indole-3-acetylglutamate are the stored auxin amino acid conjugates of the achene of the diploid strawberry and serve as sources of auxin during seedling growth.

The edible part of the strawberry, a pseudocarp, has long been known to enlarge in response to auxin produced by the developing achenes, the botanical true fruit. Auxin homeostasis involves a complex interaction between biosynthesis, conjugate formation and hydrolysis, catabolism and transport. Strawberry tissues are capable of synthesizing auxin conjugates, and transcriptome data support the expression of genes involved in IAA conjugate formation and hydrolysis throughout embryo development and subsequent seedling growth. Using a highly sensitive and selective mass spectrometric method, we identified all the low molecular weight indole-auxin amino acid conjugates in achenes of F. vesca as consisting of indole-3-acetylaspartate (IAasp) and indole-3-acetylglutamate (IAglu). In contrast to what has been proposed to occur in Arabidopsis, we determined that IAasp and IAglu are hydrolyzed by seedlings to provide a source of free IAA for growth.

Keywords

Auxin conjugates Conjugate hydrolysis IAA amidohydrolase Indole-3-acetic acid Seed auxin precursors Strawberry achenes Woodland strawberry 

Abbreviations

IAasp

Indoleacetylaspartate

IAglu

Indoleacetylglutamate

LC–MS/MS

Liquid chromatography–tandem mass spectrometry

Notes

Acknowledgements

This work was supported by USDA/ARS CRIS 8042-21220-254-00D, by Agriculture and Food Research Initiative competitive awards no. 2018-67011-28056 and 2018-67013-27503 from the USDA National Institute of Food and Agriculture, by the NSF Plant Genome Research Program grant IOS-1238812 as well as support from the Minnesota Agricultural Experiment Station and by the Gordon and Margaret Bailey Endowment for Environmental Horticulture. We thank Paul Fiesel for the synthesis of the [13C6]IAglu used in this study, Dr. Adrian Hegeman for help with figure graphics, and Dr. Laura Shannon for advice on the evolutionary analysis of the IAA amino acid conjugate hydrolases.

Supplementary material

425_2018_3061_MOESM1_ESM.pdf (98 kb)
Suppl. Fig. S1 Expression of Fragaria homologs of Arabidopsis IAA amino acid conjugate hydrolases estimated from F. vesca transcriptome data at SGR: Strawberry Genomic Resources, and F. × ananassa transcriptome data in Sánchez-Sevilla et al. (2017). Heat maps show that hydrolase genes identified in the F. vesca genome (Edger et al. 2018) are expressed in seedlings, leaves and roots, and that several of these genes are expressed in young receptacles of both species (cortex and pith for F. vesca and receptacles of F. ananassa). Hydrolases are also expressed in the achenes, the true fruits, which contain the embryo (data shown for 6–7 days after pollination for F. vesca). These heatmaps are not meant to show quantitation of gene expression but to illustrate the dynamic nature of the processes involved in auxin metabolism. Color intensity represents the reported FPKM of each gene for the tissue

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Authors and Affiliations

  1. 1.Department of Horticultural Science and Microbial and Plant Genome InstituteUniversity of MinnesotaSaint PaulUSA
  2. 2.USDA/ARS Genetic Improvement of Fruit and Vegetables LaboratoryBeltsvilleUSA

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