, Volume 249, Issue 2, pp 431–444 | Cite as

Transcriptome analysis of root-knot nematode (Meloidogyne incognita)-resistant and susceptible sweetpotato cultivars

  • Il Hwan Lee
  • Donghwan Shim
  • Jea Cheol Jeong
  • Yeon Woo Sung
  • Ki Jung Nam
  • Jung-Wook Yang
  • Joon Ha
  • Jeung Joo Lee
  • Yun-Hee KimEmail author
Original Article


Main conclusion

Transcriptome analysis was performed on the roots of susceptible and resistant sweetpotato cultivars infected with the major root-knot nematode species Meloidogyne incognita. In addition, we identified a transcription factor-mediated defense signaling pathway that might function in sweetpotato–nematode interactions.

Root-knot nematodes (RKNs, Meloidogyne spp.) are important sedentary endoparasites of many agricultural crop plants that significantly reduce production in field-grown sweetpotato. To date, no studies involving gene expression profiling in sweetpotato during RKN infection have been reported. Therefore, in the present study, transcriptome analysis was performed on the roots of susceptible (cv. Yulmi) and resistant (cv. Juhwangmi) sweetpotato cultivars infected with the widespread, major RKN species Meloidogyne incognita. Using the Illumina HiSeq 2000 platform, we generated 455,295,628 pair-end reads from the fibrous roots of both cultivars, which were assembled into 74,733 transcripts. A number of common and unique genes were differentially expressed in susceptible vs. resistant cultivars as a result of RKN infection. We assigned the differentially expressed genes into gene ontology categories and used MapMan annotation to predict their functional roles and associated biological processes. The candidate genes including hormonal signaling-related transcription factors and pathogenesis-related genes that could contribute to protection against RKN infection in sweetpotato roots were identified and sweetpotato–nematode interactions involved in resistance are discussed.


Defense signaling Root-knot nematodes Sweetpotato Transcriptome 



Differentially expressed genes




Jasmonic acid




Root-knot nematode


Salicylic acid


Transcription factor



This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Science, ICT, and Future Planning (2018R1A1A1A05018446).

Supplementary material

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Supplementary material 1 (PPTX 977 kb)
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Supplementary material 2 (XLSX 13 kb)
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Supplementary material 3 (XLSX 54 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Il Hwan Lee
    • 1
  • Donghwan Shim
    • 1
  • Jea Cheol Jeong
    • 2
  • Yeon Woo Sung
    • 3
  • Ki Jung Nam
    • 3
  • Jung-Wook Yang
    • 4
  • Joon Ha
    • 5
  • Jeung Joo Lee
    • 6
  • Yun-Hee Kim
    • 3
    Email author
  1. 1.Department of Forest Genetic ResourcesNational Institute of Forest ScienceSuwonRepublic of Korea
  2. 2.Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB)JeongeupRepublic of Korea
  3. 3.Department of Biology Education, IALSGyeongsang National UniversityJinjuRepublic of Korea
  4. 4.Bioenergy Crop Research Institute, National Institute of Crop ScienceRural Development AdministrationMuanRepublic of Korea
  5. 5.Division of Applied Life Science (BK21 Plus)Gyeongsang National UniversityJinjuRepublic of Korea
  6. 6.Department of Plant Medicine, IALSGyeongsang National UniversityJinjuRepublic of Korea

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