, Volume 248, Issue 5, pp 1319–1328 | Cite as

Fine mapping of powdery mildew resistance gene Pm4e in bread wheat (Triticum aestivum L.)

  • Khan Nasr Ullah
  • Na Li
  • Tao Shen
  • Peisi Wang
  • Wenbin Tang
  • Shengwei Ma
  • Zhimeng Zhang
  • Haiyan Jia
  • Zhongxin Kong
  • Zhengqiang MaEmail author
Original Article


Main conclusion

Fine mapping of wheat powdery mildew-resistance gene Pm4e to a 0.19 cM interval with sequence-based markers provides the foundation for map-based cloning and marker-assisted selection with breeder-friendly markers.

Powdery mildew caused by Blumeria graminis f. sp. tritici is a wheat foliar disease that poses a serious threat to global wheat production. Pm4 is a resistance gene locus that has played a key role in controlling this disease in wheat production and a few resistance alleles of this locus have been identified. We have previously mapped the Pm4e allele to a 6.7 cM interval on chromosome 2AL. In this study, Pm4e was delimited to a 0.19 cM interval flanked by Xwgrc763 and Xwgrc865, through employment of a larger segregating population, derived from the cross of resistant parent D29 with susceptible parent Yangmai 158 (Y158), and enrichment of the genetic interval with markers developed on Chinese Spring (C.S.) survey sequence. In this interval, Pm4e co-segregated with a few markers, some of which were either D29-dominant or Y158-dominant, implying great sequence variation in the interval between D29 and Y158. Most of these co-segregation markers could not differentiate the Pm4 alleles from each other. Survey of 55 wheat cultivars with four co-dominant markers showed that the Pm4e-co-segregating loci always co-exist. Annotation of the Pm4e interval-corresponding C.S. sequence revealed more than a dozen resistance gene analogs clustered in a 2.4 Mb region, although C.S. is susceptible to the Pm4e-avirulent isolate Bgt2. This study has established the foundation for map-based cloning of Pm4e. Moreover, some of the co-dominant markers developed in this study could help in marker-assisted transfer of Pm4e into elite cultivars.


Wheat Powdery mildew Chinese Spring Pm4 Fine mapping Marker-assisted selection 



This project was partially supported by National Key Research and Development Program (2016YFD0101802), NSFC Funds 30025030, 30771344, 30771165 and 31501302, Fundamental Research Fund for the Central Universities (KJQN201633), Jiangsu Collaborative Innovation Initiative for Modern Crop Production, ‘111’ project B08025, Innovation Team Program for Jiangsu Universities (2014). The first author also acknowledges the financial support from Chinese Scholarship Council for Ph. D program.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

425_2018_2990_MOESM1_ESM.xlsx (16 kb)
Supplementary material 1 Annotated genes in the C.S. interval corresponding to the Pm4e interval (XLSX 15 kb)


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018
corrected publication September/2018

Authors and Affiliations

  1. 1.The Applied Plant Genomics Laboratory of Crop Genomics and Bioinformatics CentreNanjing Agricultural UniversityNanjingChina

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