, Volume 248, Issue 5, pp 1079–1099 | Cite as

Proteomic discovery of H2O2 response in roots and functional characterization of PutGLP gene from alkaligrass

  • Juanjuan Yu
  • Yongxue Zhang
  • Junming Liu
  • Lin Wang
  • Panpan Liu
  • Zepeng Yin
  • Siyi Guo
  • Jun Ma
  • Zhuang Lu
  • Tai Wang
  • Yimin She
  • Yuchen Miao
  • Ling Ma
  • Sixue Chen
  • Ying LiEmail author
  • Shaojun DaiEmail author
Original Article


Main conclusion

Hydrogen peroxide-responsive pathways in roots of alkaligrass analyzed by proteomic studies and PutGLP enhance the plant tolerance to saline-, alkali- and cadmium-induced oxidative stresses.

Oxidative stress adaptation is critical for plants in response to various stress environments. The halophyte alkaligrass (Puccinellia tenuiflora) is an outstanding pasture with strong tolerance to salt and alkali stresses. In this study, iTRAQ- and 2DE-based proteomics approaches, as well as qRT-PCR and molecular genetics, were employed to investigate H2O2-responsive mechanisms in alkaligrass roots. The evaluation of membrane integrity and reactive oxygen species (ROS)-scavenging systems, as well as abundance patterns of H2O2-responsive proteins/genes indicated that Ca2+-mediated kinase signaling pathways, ROS homeostasis, osmotic modulation, and transcriptional regulation were pivotal for oxidative adaptation in alkaligrass roots. Overexpressing a P. tenuiflora germin-like protein (PutGLP) gene in Arabidopsis seedlings revealed that the apoplastic PutGLP with activities of oxalate oxidase and superoxide dismutase was predominantly expressed in roots and played important roles in ROS scavenging in response to salinity-, alkali-, and CdCl2-induced oxidative stresses. The results provide insights into the fine-tuned redox-responsive networks in halophyte roots.


Germin-like protein Hydrogen peroxide response Proteomics Puccinellia tenuiflora Roots Signaling 



Ascorbate peroxidase




Dehydroascorbate reductase


Germin-like protein


Glutathione peroxidase


Glutathione S-transferase


H2O2-responsive protein


Monodehydroascorbate reductase


mM H2O2 treatments for 6 h


Oxalate oxidase




Reactive oxygen species


Superoxide dismutase



The project was supported by Grants from the National Key Research and Development Program (2017YFD0600101) to Ling Ma, the Fundamental Research Funds for the Central Universities (2572017ET01, 2572017EA05, and 2572016AA16) to Shaojun Dai, Ying Li, and Juanjuan Yu, as well as the Foundation of Shanghai Science and Technology Committee, China (17391900600) to Shaojun Dai.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Juanjuan Yu
    • 1
    • 2
  • Yongxue Zhang
    • 1
    • 2
  • Junming Liu
    • 1
  • Lin Wang
    • 1
  • Panpan Liu
    • 1
  • Zepeng Yin
    • 1
  • Siyi Guo
    • 3
  • Jun Ma
    • 4
  • Zhuang Lu
    • 5
  • Tai Wang
    • 5
  • Yimin She
    • 4
  • Yuchen Miao
    • 3
  • Ling Ma
    • 1
  • Sixue Chen
    • 6
  • Ying Li
    • 1
    Email author
  • Shaojun Dai
    • 1
    • 2
    Email author
  1. 1.Alkali Soil Natural Environmental Science Center, Key Laboratory of Saline-alkali Vegetation Ecology Restoration, Ministry of EducationNortheast Forestry UniversityHarbinChina
  2. 2.Development Centre of Plant Germplasm Resources, College of Life and Environmental SciencesShanghai Normal UniversityShanghaiChina
  3. 3.Institute of Plant Stress Biology, State Key Laboratory of Cotton Biology, Department of BiologyHenan UniversityKaifengChina
  4. 4.Shanghai Center for Plant Stress BiologyChinese Academy of SciencesShanghaiChina
  5. 5.Institute of BotanyChinese Academy of SciencesBeijingChina
  6. 6.Department of Biology, Genetics Institute, Plant Molecular and Cellular Program, Interdisciplinary Center for Biotechnology ResearchUniversity of FloridaGainesvilleUSA

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