, Volume 248, Issue 3, pp 745–750 | Cite as

Magnesium uptake characteristics in Arabidopsis revealed by 28Mg tracer studies

  • Takaaki Ogura
  • Natsuko I. Kobayashi
  • Hisashi Suzuki
  • Ren Iwata
  • Tomoko M. Nakanishi
  • Keitaro Tanoi
Short Communication


Main conclusion

The Mg2+ uptake system in Arabidopsis roots is Gd3+- and Fe2+-sensitive, and responds to a changing Mg2+ concentration within 1 h with the participation of AtMRS2 transporters.


Magnesium (Mg2+) absorption and the mechanism regulating its activity have not been clarified yet. To address these issues, it is necessary to reveal the characteristics of Mg2+ uptake in roots. Therefore, we first investigated the Mg2+ uptake characteristics in roots of 1-week-old Arabidopsis plants using 28Mg. The Mg2+ uptake system in roots was up-regulated within 1 h in response to the low Mg2+ condition. This induction was inhibited in Arabidopsis “mitochondrial RNA splicing 2/magnesium transport” mutants atmrs2-4/atmgt6 and atmrs2-7/atmgt7, while the expression of AtMRS2-4/AtMGT6 and AtMRS2-7/AtMGT7 genes in the Arabidopsis wild-type was not responsive to Mg2+ conditions. In addition, the Mg deficiency-induced Mg2+ uptake system was shut-down within 5 min when Mg2+ was resupplied to the environment. An inhibition study showed that the constitutive mechanism functioning in Mg2+ uptake under Mg2+ sufficient conditions was sensitive to a number of divalent and trivalent cations, particularly Gd3+ and Fe2+, but not to K+.


Abiotic stress CorA/MRS2 family Inhibitor Mg deficiency Radionuclide Root 



Hexammine cobalt(III) chloride


Magnesium transport


Mitochondrial RNA splicing 2



Authors thank Dr. Martin O’Brien for English editing. This work was partially supported by JSPS KAKENHI Grant-in-Aid for Young Scientists (B) (number 17K15236), JSPS KAKENHI Grant-in-Aid for Scientific Research (A) (15H02469) and JST PROSTO (number JPMJPR15Q7). This work was also sponsored by JSPS and F.R.S.-FNRS under the Japan - Belgium Research Cooperative Programme.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Graduate School of Agricultural and Life SciencesThe University of TokyoTokyoJapan
  2. 2.National Institute of Radiological SciencesNational Institutes for Quantum and Radiological Science and TechnologyChibaJapan
  3. 3.Cyclotron and Radioisotope Center, Tohoku UniversitySendaiJapan
  4. 4.PRESTO, Japan Science and Technology Agency (JST)KawaguchiJapan

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