Ratio of sugar concentrations in the phloem sap and the cytosol of mesophyll cells in different tree species as an indicator of the phloem loading mechanism
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Sucrose concentration in phloem sap was several times higher than in the cytosol of mesophyll cells. The results suggest that phloem loading involves active steps in the analyzed tree species.
Phloem loading in source leaves is a key step for carbon partitioning and passive symplastic loading has been proposed for several tree species. However, experimental evidence to prove the potential for sucrose diffusion from mesophyll to phloem is rare. Here, we analyzed three tree species (two angiosperms, Fagus sylvatica, Magnolia kobus, and one gymnosperm, Gnetum gnemon) to investigate the proposed phloem loading mechanism. For this purpose, the minor vein structure and the sugar concentrations in phloem sap as well as in the subcellular compartments of mesophyll cells were investigated. The analyzed tree species belong to the open type minor vein subcategory. The sucrose concentration in the cytosol of mesophyll cells ranged between 75 and 165 mM and was almost equal to the vacuolar concentration. Phloem sap could be collected from F. sylvatica and M. kobus and the concentration of sucrose in phloem sap was about five- and 11-fold higher, respectively, than in the cytosol of mesophyll cells. Sugar exudation of cut leaves was decreased by p-chloromercuribenzenesulfonic acid, an inhibitor of sucrose–proton transporter. The results suggest that phloem loading of sucrose in the analyzed tree species involves active steps, and apoplastic phloem loading seems more likely.
KeywordsFagus sylvatica Gnetum gnemon Magnolia kobus Non-aqueous fractionation Phloem loading Phloem sap Subcellular metabolite concentration Sugar
Bundle sheath cell
The authors would like to thank Elisabeth Wesbuer and Tim Kreutzer for technical assistance, as well as Sarah Rau for help with the electron micrographs. We are grateful to Rosi Ritter and Christoph Senges for critical reading of the manuscript.
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Conflict of interest
The authors declare that they have no conflict of interest.
- Farré E, Tiessen A, Roessner U, Geigenberger P, Trethewey RN, Willmitzer L (2001) Analysis of the compartmentation of glycolytic intermediates, nucleotides, sugars, organic acids, amino acids, and sugar alcohols in potato tubers using a nonaqueous fractionation method. Plant Physiol 127:685–700CrossRefGoogle Scholar
- Karnovsky MJ (1965) A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J Cell Biol 27:137–138Google Scholar
- Lohaus G, Heldt HW (1997) Assimilation of gaseous ammonia and the transport of its products in barley and spinach leaves. J Exp Bot 48:1779–1786Google Scholar
- Münch E (1930) Die Stoffbewegungen in der Pflanze. Gustav Fischer, JenaGoogle Scholar