Survey of the occurrence of desiccation-induced quenching of basal fluorescence in 28 species of green microalgae
Desiccation-induced chlorophyll fluorescence quenching seems to be an indispensable part of desiccation resistance in the surveyed 28 green microalgal species.
Lichens are desiccation tolerant meta-organisms. In the desiccated state photosynthesis is inhibited rendering the photobionts potentially sensitive to photoinhibition. As a photoprotective mechanism, strong non-radiative dissipation of absorbed light leading to quenching of chlorophyll fluorescence has been proposed. Desiccation-induced quenching affects not only variable fluorescence, but also the so-called basal fluorescence, F0. This phenomenon is well-known for intact lichens and some free living aero-terrestrial algae, but it was often absent in isolated lichen algae. Therefore, a thorough screening for the appearance of desiccation-induced quenching was undertaken with 13 different aero-terrestrial microalgal species and lichen photobionts. They were compared with 15 aquatic green microalgal species, among them also three marine species. We asked the following questions: Do isolated lichen algae show desiccation-induced quenching? Are aero-terrestrial algae different in this respect to aquatic algae and is the potential for desiccation-induced quenching coupled to desiccation tolerance? How variable is desiccation-induced quenching among species? Most of the aero-terrestrial algae, including all lichen photobionts, showed desiccation-induced quenching, although highly variable in extent, whereas most of the aquatic algae did not. All algae displaying quenching were also desiccation tolerant, whereas all algae unable to perform desiccation-induced quenching were desiccation intolerant. Desiccation-induced fluorescence quenching seems to be an indispensable part of desiccation resistance in the investigated species.
KeywordsAero-terrestrial algae Desiccation tolerance Lichens Photobionts Photoprotection
Desiccation-induced chlorophyll fluorescence quenching
Relative air humidity
Culture Collection of Algae at the University of Göttingen, Catalogue Number
The Culture Collection of Algae at the University of Göttingen (SAG) kindly provided the algae strains. Frank-Peter Rapp and Jens Hermann helped in the construction of the apparatus for the desiccation experiments.
- Barták M, Hájek J, Gloser J (2000) Heterogeneity of chlorophyll fluorescence over thalli of several foliose macrolichens exposed to adverse environmental factors: interspecific differences as related to thallus hydration and high irradiance. Photosynthetica 38:531–537. https://doi.org/10.1023/A:1012405306648 CrossRefGoogle Scholar
- Bilger W (2014) Desiccation-induced quenching of chlorophyll fluorescence in cryptogams. In: Demmig-Adams B, Garab G, Adams WA III, Govindjee (eds) Non-photochemical quenching and energy dissipation in plants, algae and cyanobacteria. Springer Netherlands, Dordrecht, pp 409–420Google Scholar
- del Hoyo A, Álvarez R, del Campo EM, Gasulla F, Barreno E, Casano LM (2010) Oxidative stress induces distinct physiological responses in the two Trebouxia phycobionts of the lichen Ramalina farinacea. Ann Bot 107:109–118. https://doi.org/10.1093/aob/mcq206 [print version in Ann Bot 2011; epub 2010 provided by doi] CrossRefPubMedPubMedCentralGoogle Scholar
- DePriest PT (2004) Early molecular investigations of lichen-forming symbionts: 1986–2001. Annu Rev Microbiol 58:273–301. https://doi.org/10.1146/annurev.micro.58.030603.123730 CrossRefPubMedGoogle Scholar
- Dietz S, Büdel B, Lange O, Bilger W (2000) Transmittance of light through the cortex of lichens from contrasting habitats. In: Schroeter B, Schlensorg M, Green TGA (eds) New aspects in cryptogamic research: contribution in honour of Ludger Kappen. Bibliotheca Lichenologica 75:171–182Google Scholar
- Gasulla F, de Nova PG, Esteban-Carrasco A, Zapata JM, Barreno E, Guera A (2009) Dehydration rate and time of desiccation affect recovery of the lichenic algae Trebouxia erici: alternative and classical protective mechanisms. Planta 231:195–208. https://doi.org/10.1007/s00425-009-1019-y CrossRefPubMedGoogle Scholar
- Green TGA, Proctor MCF (2016) Physiology of photosynthetic organisms within biological soil crusts: their adaptation, flexibility, and plasticity. In: Weber B, Büdel B, Belnap J (eds) Biological soil crusts: an organizing principle in drylands. Springer International Publishing, Berlin, pp 347–381CrossRefGoogle Scholar
- Heber U, Bilger W, Türk R, Lange OL (2010) Photoprotection of reaction centres in photosynthetic organisms: mechanisms of thermal energy dissipation in desiccated thalli of the lichen Lobaria pulmonaria. New Phytol 185:459–470. https://doi.org/10.1111/j.1469-8137.2009.03064.x CrossRefPubMedGoogle Scholar
- Komura M, Yamagishi A, Shibata Y et al (2010) Mechanism of strong quenching of photosystem II chlorophyll fluorescence under drought stress in a lichen, Physciella melanchla, studied by subpicosecond fluorescence spectroscopy. Biochim Biophys Acta BBA Bioenerg 1797:331–338. https://doi.org/10.1016/j.bbabio.2009.11.007 CrossRefGoogle Scholar
- Krause GH, Jahns P (2004) Non-photochemical energy dissipation determined by chlorophyll fluorescence quenching: characterization and function. In: Papageorgiou GC, Govindjee (eds) Chlorophyll a fluorescence: a signature of photosynthesis. Springer Netherlands, Dordrecht, pp 463–495CrossRefGoogle Scholar
- Lange OL, Bilger W, Rimke S, Schreiber U (1989) Chlorophyll fluorescence of lichens containing green and blue-green algae during hydration by water vapor uptake and by addition of liquid water. Bot Acta 102:306–313. https://doi.org/10.1111/j.1438-8677.1989.tb00110.x CrossRefGoogle Scholar
- Osmond C (1994) What is photoinhibition? Some insights from comparisons of shade and sun plants. In: Baker NR, Bowyer JR (eds) Photoinhibition of photosynthesis from molecular mechanisms to the field. Bios Scientific Publishers, Oxford, pp 1–24Google Scholar
- SAG Database (2017) http://sagdb.uni-goettingen.de/index.php. Accessed 24 Aug 2017
- Sancho LG, Belnap J, Colesie C, Raggio J, Weber B (2016) Carbon budgets of biological soil crusts at micro-, meso-, and global scales. In: Weber B, Büdel B, Belnap J (eds) Biological soil crusts: an organizing principle in drylands. Springer International Publishing, Berlin, pp 287–304CrossRefGoogle Scholar
- Yamakawa H, van Stokkum IHM, Heber U, Itoh S (2018) Mechanisms of drought-induced dissipation of excitation energy in sun- and shade-adapted drought-tolerant mosses studied by fluorescence yield change and global and target analysis of fluorescence decay kinetics. Photosynth Res 135:285–298. https://doi.org/10.1007/s11120-017-0465-9 CrossRefPubMedGoogle Scholar