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Planta

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LBD13 positively regulates lateral root formation in Arabidopsis

  • Chuloh Cho
  • Eunkyeong Jeon
  • Shashank K. Pandey
  • Se Hoon Ha
  • Jungmook KimEmail author
Short Communication
  • 130 Downloads

Abstract

Main conclusion

Lateral Organ Boundaries Domain 13 (LBD13), which is expressed in emerged lateral roots and encodes a transcriptional activator, plays an important role in lateral root formation in Arabidopsis.

Lateral roots (LRs) are major determinants of root system architecture, contributing to the survival strategies of plants. Members of the LBD gene family encode plant-specific transcription factors that play key roles in plant organ development. Several LBD genes, such as LBD14, 16, 18, 29, and 33, have been shown to play important roles in regulating LR development in Arabidopsis. In the present study, we show that LBD13 is expressed in emerged LRs and LR meristems of elongated LRs and regulates LR formation in Arabidopsis. Transient gene expression assays with Arabidopsis protoplasts showed that LBD13 is localized to the nucleus and harbors transcription-activating potential. Knock-down of LBD13 expression by RNA interference resulted in reduced LR formation, whereas overexpression of LBD13 enhanced LR formation in transgenic Arabidopsis. Analysis of β-glucuronidase (GUS) expression under the control of the LBD13 promoter showed that GUS staining was detected in LRs emerged from the primary root, but not in LR primordia. Moreover, both the distribution of LR primordium number and developmental kinetics of LR primordia were not affected either by knock-down or by overexpression of LBD13. Taken together, these results suggest that LBD13 is a nuclear-localized transcriptional activator and controls LR formation during or after LR emergence.

Keywords

Arabidopsis thaliana Lateral root Lateral organ boundaries domain Transcription factor LBD14 Protoplasts RNAi 

Abbreviations

ARF

Auxin response factor

Aux/IAA

Auxin/indole 3-acetic acid protein

GUS

β-Glucuronidase

LBD

Lateral organ boundaries domain

LR

Lateral root

LRP

Lateral root primordium

PGI

Post-gravitropic induction

Notes

Acknowledgements

This work was supported by Grants to J. Kim from the Next-Generation BioGreen 21 Program (PJ013220), Rural Development Administration, Republic of Korea, and the Mid-Career Researcher Program (2016R1A2B4015201) and the Basic Research Laboratory (2017R1A4A1015620) through the National Research Foundation of Korea, funded by the Ministry of Education, Science, and Technology of Korea.

Supplementary material

425_2018_3087_MOESM1_ESM.xlsx (16 kb)
Supplementary material 1 (XLSX 17 kb)
425_2018_3087_MOESM2_ESM.pdf (557 kb)
Supplementary material 2 (PDF 557 kb)

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of Bioenergy Science and TechnologyChonnam National UniversityGwangjuSouth Korea
  2. 2.Kumho Life Science LaboratoryChonnam National UniversityGwangjuSouth Korea

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