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Cloning and characterization of a monoterpene synthase gene from flowers of Camelina sativa

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Abstract

Main conclusion

CsTPS1 encodes for a monoterpene synthase that contributes to the emission of a blend of volatile compounds emitted from flowers of Camelina sativa.

The work describes the in vitro characterization of a monoterpene synthase and its regulatory region that we cloned from Camelina sativa (Camelina). Here, we named this gene as C. sativa terpene synthase 1 (CsTPS1). In vitro experiments performed with the CsTPS1 protein after expression and purification from Escherichia coli (E. coli) showed production of a blend of monoterpene volatile organic compounds, of which the emission was also detected in the floral bouquet of wild-type Camelina plants. Quantitative-PCR measurements revealed a high abundance of CsTPS1 transcripts in flowers and experiments performed with the GUS reporter showed high CsTPS1 expression in the pistil, in the cells of the wall of the ovary and in the stigma. Subcellular localization of the CsTPS1 protein was investigated with a GFP reporter construct that showed expression in plastids. The CsTPS1 gene identified in this study belongs to a mid-size family of 60 genes putatively codifying for TPS enzymes. This enlarged family of TPS genes suggests that Camelina has the structural framework for the production of terpenes and other secondary metabolites of relevance for the consumers.

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Change history

  • 13 November 2017

    In the original publication, the order of figures and citations was incorrect. The corrections are listed below:

Abbreviations

GPP:

Geranyl pyrophosphate

VOCs:

Volatiles organic compounds

TPS:

Terpene synthase

PTP:

Plastid targeting peptide

MVA:

Mevalonate

MEP:

2-C-Methyl-d-erythritol 4-phosphate

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Acknowledgements

We are grateful to Jing Xi for providing the plasmid containing the sequence of the small subunit of the RuBisco and to Eva Johannes for her support with the confocal analysis. This research was supported by the Advanced Research Projects Agency-Energy (ARPA-E Grant no. 554667-06858 to De-Yu Xie).

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Correspondence to De-Yu Xie.

Additional information

The original version of this article was revised: In the original publication, the order of figures and citations was incorrect and it has been corrected now.

A correction to this article is available online at https://doi.org/10.1007/s00425-017-2810-9.

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Borghi, M., Xie, D. Cloning and characterization of a monoterpene synthase gene from flowers of Camelina sativa . Planta 247, 443–457 (2018). https://doi.org/10.1007/s00425-017-2801-x

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Keywords

  • Terpene Synthase
  • Flower
  • Terpenoids
  • Volatile Organic Compounds
  • Camelina