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Involvement of nitrite in the nitrate-mediated modulation of fermentative metabolism and nitric oxide production of soybean roots during hypoxia

Abstract

It is widely accepted that nitrate but not ammonium improves tolerance of plants to hypoxic stress, although the mechanisms related to this beneficial effect are not well understood. Recently, nitrite derived from nitrate reduction has emerged as the major substrate for the synthesis of nitric oxide (NO), an important signaling molecule in plants. Here, we analyzed the effect of different nitrogen sources (nitrate, nitrite and ammonium) on the metabolic response and NO production of soybean roots under hypoxia. Organic acid analysis showed that root segments isolated from nitrate-cultivated plants presented a lower accumulation of lactate and succinate in response to oxygen deficiency in relation to those from ammonium-cultivated plants. The more pronounced lactate accumulation by root segments of ammonium-grown plants was followed by a higher ethanol release in the medium, evidencing a more intense fermentation under oxygen deficiency than those from nitrate-grown plants. As expected, root segments from nitrate-cultivated plants produced higher amounts of nitrite and NO during hypoxia compared to ammonium cultivation. Exogenous nitrite supplied during hypoxia reduced both ethanol and lactate production and stimulated cyanide-sensitive NO emission by root segments from ammonium-cultivated plants, independent of nitrate. On the other hand, treatments with a NO donor or a NO scavenger did not affect the intensity of fermentation of soybean roots. Overall, these results indicate that nitrite participates in the nitrate-mediated modulation of the fermentative metabolism of soybean roots during oxygen deficiency. The involvement of mitochondrial reduction of nitrite to NO in this mechanism is discussed.

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Abbreviations

cPTIO:

2-(4-Carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl-3-oxide

DAF-2:

4,5-Diaminofluorescein

GC–MS:

Gas chromatography–mass spectrometry

NH4 + :

Ammonium

NO:

Nitric oxide

NO2 :

Nitrite

NO3 :

Nitrate

NOC-5:

3-(Aminopropyl)-1-hydroxy-3-isopropyl-2-oxo-1-triazene

NR:

Nitrate reductase

O2 :

Molecular oxygen

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Acknowledgments

The authors wish to thank Dr. Fernanda Ramos Gadelha for the use of the spectrofluorometer. This work was supported by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, grant nº 303931/2009-4). H.C.O. was supported by a fellowship from the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, Grant No. 2009/17583-3).

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Correspondence to Halley C. Oliveira.

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Oliveira, H.C., Salgado, I. & Sodek, L. Involvement of nitrite in the nitrate-mediated modulation of fermentative metabolism and nitric oxide production of soybean roots during hypoxia. Planta 237, 255–264 (2013). https://doi.org/10.1007/s00425-012-1773-0

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Keywords

  • Ammonium
  • Fermentation
  • Nitrate
  • Nitric oxide
  • Nitrite
  • Oxygen deficiency