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Glandular trichomes of Ceratotheca triloba (Pedaliaceae): morphology, histochemistry and ultrastructure

Abstract

This study was initiated to characterize the distribution, morphology, secretion mode, histochemistry and ultrastructure of the glandular trichomes of Ceratotheca triloba using light and electron microscopy. Its leaves bear two morphologically distinct glandular trichomes. The first type has long trichome with 8–12 basal cells of pedestal, 3–14 stalk cells, a neck cell and a head of four cells in one layer. The second type has short trichome comprising one or two basal epidermal cells, a unicellular or bicellular stalk and a multicellular head of two to eight cells. There is a marked circular area in the upper part of each head cell of the long trichome. This area is provided with micropores to exudate directly the secretory product onto the leaf surface by an eccrine pathway. The secretory product has copious amount of dark microbodies arising from plastids which are positive to Sudan tests and osmium tetroxide for unsaturated lipids. The secretion mode of short trichomes is granulocrine and involves two morphologically and histochemically distinct vesicle types: small Golgi-derived vesicles which are positive to Ruthenium Red test for mucilaginous polysaccharides; the second type is dark large microbodies similar to that of long trichomes with low quantity. These two types are stored in numerous peripheral vacuoles and discharge their contents accompanied by the formation of irregular invaginations of the plasmalemma inside the vacuoles via reverse pinocytosis. These two secretion modes of long and short trichomes are reported for the first time in the family Pedaliaceae. The long trichomes have more unsaturated lipids, while the short trichomes contain more mucilaginous polysaccharides.

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References

  1. Abels J (1975) Monographs of the African Pedaliaceae. Memórias da Sociedade Broteriana 25, Coimbra

  2. Adjanohoun EJ (1986) Contributions aux Etudes Ethnobotaniqueset Floristiquesa u Togo. Medecine Traditionellee t Pharmacopee. A gence de Cooperation Culturelle et Technique, Paris

  3. Ascensão L, Pais MS (1987) Glandular trichomes of Artemisia campestris (ssp. maritima): ontogeny and histochemistry of the secretory product. Bot Gaz 148:221–227

  4. Ascensao L, Figueiredo AC, Barroso JG, Pedro LG, Schripsema J, Deans SG, Scheffer JJC (1998) Plectranthus madagascariensis: morphology of the glandular trichomes, essential oil composition, and its biological activity. Int J Plant Sci 159(1):31–38

  5. Ascensão L, Mota L, Castro M (1999) Glandular trichomes on the leaves and flowers of Plectranthus ornatus: morphology, distribution and histochemistry. Ann Bot 84:437–447

  6. Bornman CH, Spurr AR, Addicott FT (1969) Histochemical localization by electron microscopy of pectic substances in abscising tissue. S Afr J Bot 35:253

  7. Bosabalidis A, Tsekos I (1982) Ultrastructural studies on the secretory cavities of Citrus deliciosa Ten. II. Development of the oil-accumulating central space of the gland and process of active secretion. Protoplasma 112:63–70

  8. Brundrett MC, Kendrick B, Peterson CA (1991) Efficient lipid staining in plant material with Sudan Red 7B or fluoral yellow 088 in polyethylene glycol–glycerol. Biotech Histochem 66:111–116

  9. Clausen J, Keck DD, Hiesey WM (1940) Experimental studies on the nature of species. I. Effect of varied environments on Western North American plants. Publication 520. Carnegie Institution of Washington, Washington, DC

  10. Cronquist A (1981) An integrated system of classification of flowering plants. Columbia University Press, New York

  11. David R, Carde JP (1964) Coloration différentielle des inclusions lipidiqueet terpéniques des pseudophylles du pin maritime au moyen du réactif Nadi. Comptes Rendus de l’ Académie des Sciences, Paris 258:1338–1340

  12. Fahn A (1979) Secretory tissues in plants. Academic Press, London, pp 158–221

  13. Fahn A (1988) Secretory tissues in plants. New Phytol 108(3):229–257

  14. Fahn A, Evert RF (1974) Ultrastructure of the secretory ducts of Rhus glabra L. Am J Bot 61:1–14

  15. Feder N, O’Brien TP (1968) Plant microtechnique: some principles and new methods. Am J Bot 55:123–142

  16. Fernandes GW (1994) Plant mechanical defences against insect herbivory. Revista Brasileira de Entomologia 38:421–433

  17. Furr M, Mahlberg PG (1981) Histochemical analyses of laticifers and glandular trichomes in Cannabis sativa. J Nat Prod 44:153–159

  18. Gabe M (1968) Techniques histologiques. Masson and Cie, Paris 241

  19. Gaff DF (1997) Mechanisms of desiccation tolerance in resurrection vascular plants. In: Basra AS, Basra RK (eds) Mechanisms of environmental stress resistance in plants. Harwood Academic Publishers, Amsterdam, pp 43–58

  20. Gibson RW (1971) Glandular hairs providing resistance to aphids in certain wild potato species. Ann Appl Biol 68:113–119

  21. Hardman R, Sofowara EA (1972) Antimonium trichloride as a test reagent for steroids especially diosgenin and yamogenin in plant tissues. Stain Technol J 47:205–208

  22. Ihlenfeldt HD (2001) Fitting pieces together Pterodiscus Hooker (Pedaliaceae) in tropical NE Africa, A case study. In: Friis I, Ryding O (eds) Biodiversity research in the horn of Africa region. The Royal Danish Academy of Sciences and Letters, Copenhagen, pp 63–74

  23. Jensen WA (1962) Botanical histochemistry: principles and practice. WH Freeman & Co, San Francisco

  24. Joel DM, Fahn A (1980) Ultrastructure of resin ducts of Mangifera indica L. (Anacardiaceae). 3. Secretion of protein–polysaccharide mucilage in the fruit. Ann Bot 46:785–790

  25. Johansen DA (1940) Plant microtechnique. McGraw-Hill, New York

  26. Johnson HB (1975) Plant pubescence: an ecological perspective. Bot Rev 41:233–258

  27. Lison L (1960) Histochemieetcytochemie animals. Principesetméthods 1(2), Gauthier-Villars, Paris

  28. Mabry TJ, Markham KR, Thomas MB (1970) The systematic identification of flavonoids. Springer, Berlin

  29. Mace ME, Bell AA, Stipanovic RD (1974) Histochemistry and isolation of gossypol and related terpenoids in roots of cotton seedlings. Phytopathology 64:1297–1302

  30. Mahlberg P, Hammond CT, Turner JC, Hemphill JK (1984) Structure, development and composition of glandular trichomes of Cannabis sativa L. In: Rodriguez E, Healey PL, Mehta I (eds) Biology and chemistry of plant trichomes. Plenum Press, New York, pp 23–51

  31. Metcalfe CR, Chalk L (1972) Anatomy of the dicotyledons: leaves, stem and wood in relation to taxonomy with notes on economic uses, vol II, 4th edn. Oxford University Press, London, pp 1013–1014

  32. Modenesi P, Serato-Valenti G, Bruni A (1984) Development and secretion of clubbed trichomes in Thymus vulgaris L. Flora 175:211–219

  33. Pearse AGE (1985) Histochemistry theoretical and applied, 4th edn. Churchill Livingstone, London

  34. Reynolds ES (1963) The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J Cell Biol 17:208–212

  35. Schnepf E, Deichgraber G, Barthlott W (1983) On the fine structure of the liquid producing floral gland of the orchid, Coryanthes speciosa. Nordic J Bot 3:479–491

  36. Solereder H (1908) Systematic anatomy of the dicotyledons, vol I. Clarendon Press, Oxford, pp 611–613

  37. Spurr AR (1969) A low-viscosity epoxy resin embedding medium for electron microscopy. J Ultrastructural Res 26:31–43

  38. Tingey WM, Gibson WR (1978) Feeding and mobility of the potato leafhopper impaired by glandular trichomes of Solanum berthaultii and S. polyadenium. J Econ Entomol 71:856–858

  39. Todd WJ (1986) Effects of specimen preparation on the apparent ultrastructure of microorganisms. In: Aldrich HC, Todd WJ (eds) Ultrastructure techniques for microorganisms. Plenum Press, New York, pp 87–99

  40. Trachtenberg S, Fahn A (1981) The mucilage cells of Opuntia ficus-indica (L) Mill: development, ultrastructure, and mucilage secretion. Bot Gaz 142:206–213

  41. Venkatachalam KV, Kjonaas R, Croteau R (1984) Development and essential oil content of secretory glands of sage (Salvia officianalis). Plant Physiol 76:148–150

  42. Werker E (2000) Trichome diversity and development. In: Hallahan DL, Gray JC (eds) Advances in botanical research: plant trichormes, vol 31. Academic Press, San Diego, pp 1–35

  43. Werker E, Ravid U, Putievsky E (1985) Structure of glandular hairs and identification of the main components of their secreted material in some species of the Labiatae. Isr J Bot 34:31–45

  44. Werker E, Putievsky E, Ravid U, Dudai N, Katzir I (1993) Glandular hairs and essential oil in developing leaves of Ocimum basilicum L. (Lamiaceae). Ann Bot 71:43–50

  45. Wood JRI, Haig-Thomas H (1997) A hand-book of the flora of Yemen. Royal Botanic Gardens, Kew

  46. Woodman RL, Fernandes GW (1991) Differential mechanical defense: herbivory, evapotranspiration, and leaf hairs. Oikos 60:11–19

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Acknowledgment

The authors gratefully acknowledge the National Research Foundation, South Africa, for financial support.

Author information

Correspondence to Yougasphree Naidoo or Samia Heneidak.

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Naidoo, Y., Karim, T., Heneidak, S. et al. Glandular trichomes of Ceratotheca triloba (Pedaliaceae): morphology, histochemistry and ultrastructure. Planta 236, 1215–1226 (2012). https://doi.org/10.1007/s00425-012-1671-5

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Keywords

  • Foxglove
  • Unsaturated lipids
  • Long trichomes
  • Medicinal plant
  • Microbodies
  • Mucilaginous polysaccharides
  • Secretion mode
  • Short trichomes
  • Vesicles