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Stimulation history affects vasomotor responses in rat mesenteric arterioles

  • Bjørn Olav Hald
  • Rasmus B. Sørensen
  • Preben G. Sørensen
  • Charlotte M. Sørensen
  • Jens Chr. Brings Jacobsen
Integrative Physiology
Part of the following topical collections:
  1. Integrative Physiology

Abstract

Resistance vessels regulate blood flow by continuously adjusting activity of the wall smooth muscle cells. These cells integrate a variety of stimuli from blood, endothelium, autonomic nerves, and surrounding tissues. Each stimulus elicits an intracellular signaling cascade that eventually influences activation of the contractile machinery. The characteristic time scale of each cascade and the sharing of specific reactions between cascades provide for complex behavior when a vessel receives multiple stimuli. Here, we apply sequential stimulation with invariant concentrations of vasoconstrictor (norepinephrine/methoxamine) and vasodilator (SNAP/carbacol) to rat mesenteric vessels in the wire myograph to show that (1) time elapsed between addition of two vasoactive drugs and (2) the sequence of addition may significantly affect final force development. Furthermore, force oscillations (vasomotion) often appear upon norepinephrine administration. Using computational modeling in combination with nitric oxide (NO) inhibition/NO addition experiments, we show that (3) amplitude and number of oscillating vessels increase over time, (4) the ability of NO to induce vasomotion depends on whether it is applied before or after norepinephrine, and (5) emergence of vasomotion depends on the prior dynamical state of the system; in simulations, this phenomenon appears as “hysteresis.” These findings underscore the time-dependent nature of vascular tone generation which must be considered when evaluating the vasomotor effects of multiple, simultaneous stimuli in vitro or in vivo.

Keywords

Contractile state Vasomotion Mesenteric artery Non-linear dynamics 

Abbreviations

EC

Endothelial cell

SMC

Smooth muscle cell

cpm

Cycles per minute

PSS

Physiological saline solution

NE

Norepinephrine

MO

Methoxamine

SNAP

S-Nitroso-N-acetyl-DL-penicillamine

CB

Carbachol

Notes

Funding information

BOH is supported by the Danish Council for Independent Research (DFF – 1333-00172) and Weimann-fonden.

Compliance with ethical standards

All experimental protocols conformed to the European Convention for the Protection of Vertebrate Animals used for Experimental and other Scientific Purposes and were approved by the Danish National Animal Experiments Inspectorate.

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of NeuroscienceUniversity of CopenhagenCopenhagenDenmark
  2. 2.Department of Biomedical SciencesUniversity of CopenhagenCopenhagenDenmark
  3. 3.Department of ChemistryUniversity of CopenhagenCopenhagenDenmark

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