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Impact of a 10 km running trial on eryptosis, red blood cell rheology, and electrophysiology in endurance trained athletes: a pilot study

  • Elie Nader
  • David Monedero
  • Mélanie Robert
  • Sarah Skinner
  • Emeric Stauffer
  • Agnès Cibiel
  • Michèle Germain
  • Jules Hugonnet
  • Alexander Scheer
  • Philippe Joly
  • Céline Renoux
  • Philippe ConnesEmail author
  • Stéphane Égée
Original Article
  • 42 Downloads

Abstract

Purpose

Blood rheology is a key determinant of blood flow and tissue perfusion. There are still large discrepancies regarding the effects of an acute running exercise on blood rheological properties and red blood cell (RBC) physiology. We investigated the effect of a 10 km running trial on markers of blood rheology and RBC physiology in endurance trained athletes.

Methods

Blood was sampled before and after the exercise to measure lactate and glucose, hematological and hemorheological parameters (blood viscosity, RBC deformability, and aggregation), eryptosis markers (phosphatidylserine and CD47 exposure, RBC reactive oxygen species), RBC-derived microparticles (RBC-MPs), and RBC electrophysiological activity. Weight was measured before and after exercise. Peripheral oxygen saturation and heart rate were monitored before and during the trial.

Results

Blood lactate and glucose levels increased after exercise and subjects significantly lost weight. All athletes experienced a significant fall in oxygen saturation. Mean corpuscular volume (MCV) was increased from 95.1 ± 3.2 to 96.0 ± 3.3 and mean corpuscular hemoglobin concentration (MCHC) decreased after exercise suggesting a slight RBC rehydration. Exercise increased RBC deformability from 0.344 ± 0.04 to 0.378 ± 0.07, decreased RBC aggregates strength and blood viscosity, while hematocrit (Hct) remained unaffected. While RBC electrophysiological recording suggested a modulation in RBC calcium content and/or chloride conductance, eryptosis markers and RBC-MPs were not modified by the exercise.

Conclusion

A 10 km acute running exercise had no effect on RBC senescence and membrane blebbing. In contrast, this exercise increased RBC deformability, probably through rehydration process which resulted in a decrease in blood viscosity.

Keywords

Running exercise Red blood cell Rheology Eryptosis Microparticles 

Abbreviations

Ca2+

Calcium

CCCP

Carbonyl cyanide m-chlorophenylhydrazone

EI

Elongation index

Hb

Hemoglobin

Hct

Hematocrit

HR

Heart rate

Hypermax

Red blood cell maximum hyperpolarization

MAS

Maximal aerobic speed

MCH

Mean corpuscular hemoglobin

MCHC

Mean corpuscular hemoglobin concentration

MCV

Mean corpuscular volume

MFI

Median fluorescence intensity

MPs

Microparticles

NS

Not significant

PLA

Platelets

PS

Phosphatidylserine

RBC

Red blood cells

ROS

Reactive oxygen species

RT

Room temperature

SPO2

Peripheral oxygen saturation

S/V

Surface to volume ratio

Vm

Membrane potential

VO2 max

Maximal oxygen consumption

WBC

White blood cells

Notes

Author contributions

All the authors approved the final version of the manuscript. EN, PC, DM, SE, MR, SS performed research, analyzed the data, and wrote the manuscript. ES, JH, and MG performed research. CR, PJ, AC, and AS edited the manuscript.

Funding

No funding was received for this study.

Compliance with ethical standards

Conflict of interest

None of the authors have any conflict of interest.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Elie Nader
    • 1
    • 2
  • David Monedero
    • 2
    • 3
  • Mélanie Robert
    • 1
    • 2
    • 4
  • Sarah Skinner
    • 1
    • 2
  • Emeric Stauffer
    • 1
    • 2
    • 5
  • Agnès Cibiel
    • 4
  • Michèle Germain
    • 1
    • 2
    • 6
  • Jules Hugonnet
    • 6
  • Alexander Scheer
    • 4
  • Philippe Joly
    • 1
    • 2
    • 7
  • Céline Renoux
    • 1
    • 2
    • 7
  • Philippe Connes
    • 1
    • 2
    • 8
    Email author
  • Stéphane Égée
    • 2
    • 3
  1. 1.Laboratoire Interuniversitaire de Biologie de la Motricité (LIBM) EA7424, Team “Vascular Biology and Red Blood Cell”Université Claude Bernard Lyon 1, Université de LyonLyonFrance
  2. 2.Laboratoire d’Excellence du Globule Rouge (Labex GR-Ex)PRES SorbonneParisFrance
  3. 3.UMR 8227 CNRS-Sorbonne Université, Station BiologiqueRoscoff CedexFrance
  4. 4.Erytech PharmaLyonFrance
  5. 5.Centre de Médecine du Sommeil et des Maladies RespiratoiresHôpital Croix Rousse, Hospices Civils de LyonLyonFrance
  6. 6.Service d’Exploration Fonctionnelle RespiratoireHospices Civils de Lyon, Hôpital Croix RousseLyonFrance
  7. 7.Laboratoire de Biochimie et de Biologie Moléculaire, UF de Biochimie des Pathologies Érythrocytaires, Centre de Biologie et de Pathologie EstHospices Civils de LyonLyonFrance
  8. 8.Institut Universitaire de FranceParisFrance

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