Impact of a 10 km running trial on eryptosis, red blood cell rheology, and electrophysiology in endurance trained athletes: a pilot study
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Blood rheology is a key determinant of blood flow and tissue perfusion. There are still large discrepancies regarding the effects of an acute running exercise on blood rheological properties and red blood cell (RBC) physiology. We investigated the effect of a 10 km running trial on markers of blood rheology and RBC physiology in endurance trained athletes.
Blood was sampled before and after the exercise to measure lactate and glucose, hematological and hemorheological parameters (blood viscosity, RBC deformability, and aggregation), eryptosis markers (phosphatidylserine and CD47 exposure, RBC reactive oxygen species), RBC-derived microparticles (RBC-MPs), and RBC electrophysiological activity. Weight was measured before and after exercise. Peripheral oxygen saturation and heart rate were monitored before and during the trial.
Blood lactate and glucose levels increased after exercise and subjects significantly lost weight. All athletes experienced a significant fall in oxygen saturation. Mean corpuscular volume (MCV) was increased from 95.1 ± 3.2 to 96.0 ± 3.3 and mean corpuscular hemoglobin concentration (MCHC) decreased after exercise suggesting a slight RBC rehydration. Exercise increased RBC deformability from 0.344 ± 0.04 to 0.378 ± 0.07, decreased RBC aggregates strength and blood viscosity, while hematocrit (Hct) remained unaffected. While RBC electrophysiological recording suggested a modulation in RBC calcium content and/or chloride conductance, eryptosis markers and RBC-MPs were not modified by the exercise.
A 10 km acute running exercise had no effect on RBC senescence and membrane blebbing. In contrast, this exercise increased RBC deformability, probably through rehydration process which resulted in a decrease in blood viscosity.
KeywordsRunning exercise Red blood cell Rheology Eryptosis Microparticles
Carbonyl cyanide m-chlorophenylhydrazone
Red blood cell maximum hyperpolarization
Maximal aerobic speed
Mean corpuscular hemoglobin
Mean corpuscular hemoglobin concentration
Mean corpuscular volume
Median fluorescence intensity
Red blood cells
Reactive oxygen species
Peripheral oxygen saturation
Surface to volume ratio
- VO2 max
Maximal oxygen consumption
White blood cells
All the authors approved the final version of the manuscript. EN, PC, DM, SE, MR, SS performed research, analyzed the data, and wrote the manuscript. ES, JH, and MG performed research. CR, PJ, AC, and AS edited the manuscript.
No funding was received for this study.
Compliance with ethical standards
Conflict of interest
None of the authors have any conflict of interest.
- Baskurt OK, Boynard M, Cokelet GC, Connes P, Cooke BM, Forconi S, Liao F, Hardeman MR, Jung F, Meiselman HJ, Nash G, Nemeth N, Neu B, Sandhagen B, Shin S, Thurston G, Wautier JL, International Expert Panel for Standardization of Hemorheological M (2009) New guidelines for hemorheological laboratory techniques. Clin Hemorheol Microcirc 42(2):75–97. https://doi.org/10.3233/ch-2009-1202 CrossRefPubMedGoogle Scholar
- Bennekou P, de Franceschi L, Pedersen O, Lian L, Asakura T, Evans G, Brugnara C, Christophersen P (2001) Treatment with NS3623, a novel Cl-conductance blocker, ameliorates erythrocyte dehydration in transgenic SAD mice: a possible new therapeutic approach for sickle cell disease. Blood 97(5):1451–1457CrossRefGoogle Scholar
- Cabrales P, Martini J, Intaglietta M, Tsai AG (2006) Blood viscosity maintains microvascular conditions during normovolemic anemia independent of blood oxygen-carrying capacity. Am J Physiol Heart Circ Physiol 291(2):H581–H590. https://doi.org/10.1152/ajpheart.01279.2005 CrossRefPubMedGoogle Scholar
- Cokelet GR, Meiselman HJ (2007) Macro- and micro-rheological properties of blood. In: Baskurt OK, Hardeman MR, Rampling MW, Meiselman HJ (eds) Handbook of hemorheology and hemodynamics, pp 45–71. ISBN 978-1-58603-771-0Google Scholar
- Connes P, Bouix D, Py G, Caillaud C, Kippelen P, Brun JF, Varray A, Prefaut C, Mercier J (2004a) Does exercise-induced hypoxemia modify lactate influx into erythrocytes and hemorheological parameters in athletes? J Appl Physiol 97(3):1053–1058. https://doi.org/10.1152/japplphysiol.00993.2003 CrossRefPubMedGoogle Scholar
- Connes P, Frank S, Martin C, Shin S, Aufradet E, Sunoo S, Klara B, Raynaud de Mauverger E, Romana M, Messonnier L, Kang J, Varlet-Marie E, Feasson L, Hardy-Dessources MD, Wilhelm B, Brun JF (2010) New fundamental and applied mechanisms in exercise hemorheology. Clin Hemorheol Microcirc 45(2–4):131–141. https://doi.org/10.3233/CH-2010-1291 CrossRefPubMedGoogle Scholar
- Gay F, Aguera K, Senechal K, Tainturier A, Berlier W, Maucort-Boulch D, Honnorat J, Horand F, Godfrin Y, Bourgeaux V (2017) Methionine tumor starvation by erythrocyte-encapsulated methionine gamma-lyase activity controlled with per os vitamin B6. Cancer Med 6(6):1437–1452. https://doi.org/10.1002/cam4.1086 CrossRefPubMedCentralGoogle Scholar
- Makhro A, Haider T, Wang J, Bogdanov N, Steffen P, Wagner C, Meyer T, Gassmann M, Hecksteden A, Kaestner L, Bogdanova A (2016) Comparing the impact of an acute exercise bout on plasma amino acid composition, intraerythrocytic Ca(2 +) handling, and red cell function in athletes and untrained subjects. Cell Calcium 60(4):235–244. https://doi.org/10.1016/j.ceca.2016.05.005 CrossRefPubMedGoogle Scholar
- Nader E, Guillot N, Lavorel L, Hancco I, Fort R, Stauffer E, Renoux C, Joly P, Germain M, Connes P (2018) Eryptosis and hemorheological responses to maximal exercise in athletes: comparison between running and cycling. Scand J Med Sci Sports 28(5):1532–1540. https://doi.org/10.1111/sms.13059 CrossRefPubMedGoogle Scholar
- Nebor D, Romana M, Santiago R, Vachiery N, Picot J, Broquere C, Chaar V, Doumdo L, Odievre MH, Benkerrou M, Elion J (2013) Fetal hemoglobin and hydroxycarbamide modulate both plasma concentration and cellular origin of circulating microparticles in sickle cell anemia children. Haematologica 98(6):862–867. https://doi.org/10.3324/haematol.2012.073619 CrossRefPubMedPubMedCentralGoogle Scholar
- Renoux C, Faivre M, Bessaa A, Da Costa L, Joly P, Gauthier A, Connes P (2019) Impact of surface-area-to-volume ratio, internal viscosity and membrane viscoelasticity on red blood cell deformability measured in isotonic condition. Sci Rep 9(1):6771. https://doi.org/10.1038/s41598-019-43200-y CrossRefPubMedPubMedCentralGoogle Scholar
- Robach P, Boisson RC, Vincent L, Lundby C, Moutereau S, Gergele L, Michel N, Duthil E, Feasson L, Millet GY (2014) Hemolysis induced by an extreme mountain ultra-marathon is not associated with a decrease in total red blood cell volume. Scand J Med Sci Sports 24(1):18–27. https://doi.org/10.1111/j.1600-0838.2012.01481.x CrossRefPubMedGoogle Scholar
- Strobaek D, Teuber L, Jorgensen TD, Ahring PK, Kjaer K, Hansen RS, Olesen SP, Christophersen P, Skaaning-Jensen B (2004) Activation of human IK and SK Ca2 + -activated K + channels by NS309 (6,7-dichloro-1H-indole-2,3-dione 3-oxime). Biochim Biophys Acta 1665(1–2):1–5. https://doi.org/10.1016/j.bbamem.2004.07.006 CrossRefPubMedGoogle Scholar
- Tomschi F, Bizjak D, Bloch W, Latsch J, Predel HG, Grau M (2018) Deformability of different red blood cell populations and viscosity of differently trained young men in response to intensive and moderate running. Clin Hemorheol Microcirc 69(4):503–514. https://doi.org/10.3233/CH-189202 CrossRefPubMedGoogle Scholar
- Tripette J, Hardy-Dessources MD, Beltan E, Sanouiller A, Bangou J, Chalabi T, Chout R, Hedreville M, Broquere C, Nebor D, Dotzis G, Hue O, Connes P (2011) Endurance running trial in tropical environment: a blood rheological study. Clin Hemorheol Microcirc 47(4):261–268. https://doi.org/10.3233/CH-2011-1388 CrossRefGoogle Scholar
- Tsai AG, Cabrales P, Intaglietta M (2005) Blood viscosity: a factor in tissue survival? Crit Care Med 33(7):1662–1663. https://doi.org/10.1097/01.ccm.0000170177.64555.db CrossRefGoogle Scholar
- Waltz X, Hardy-Dessources MD, Lemonne N, Mougenel D, Lalanne-Mistrih ML, Lamarre Y, Tarer V, Tressieres B, Etienne-Julan M, Hue O, Connes P (2015) Is there a relationship between the hematocrit-to-viscosity ratio and microvascular oxygenation in brain and muscle? Clin Hemorheol Microcirc 59(1):37–43. https://doi.org/10.3233/CH-131742 CrossRefPubMedGoogle Scholar
- Zarychanski R, Schulz VP, Houston BL, Maksimova Y, Houston DS, Smith B, Rinehart J, Gallagher PG (2012) Mutations in the mechanotransduction protein PIEZO1 are associated with hereditary xerocytosis. Blood 120(9):1908–1915. https://doi.org/10.1182/blood-2012-04-422253 CrossRefPubMedPubMedCentralGoogle Scholar