Consecutive days of exercise decrease insulin response more than a single exercise session in healthy, inactive men
It is reported that a single bout of exercise can lower insulin responses 12–24 h post-exercise; however, the insulin responses to alternate or consecutive bouts of exercise is unknown. Thus, the purpose of this study was to examine the effect of exercise pattern on post-exercise insulin and glucose responses following a glucose challenge.
Ten male participants (n = 10, mean ± SD, Age 29.5 ± 7.7 years; BMI 25.7 ± 3.0 kg/m2) completed three exercise trials of walking for 60 min at ~ 70% of VO2max. The trials consisted of: three consecutive exercise days (3CON), three alternate exercise days (3ALT), a single bout of exercise (SB), and a no exercise control (R). Twelve to fourteen hours after the last bout of exercise or R, participants completed a 75 g oral glucose tolerance test (OGTT) and blood was collected at 30 min intervals for the measurement of glucose, insulin, and C-peptide.
Calculated incremental area under the curve (iAUC) for glucose and C-peptide was not different between the four trials. Insulin iAUC decreased 34.9% for 3CON compared to R (p < 0.01).
Three consecutive days of walking at ~ 70% VO2max improved insulin response following an OGTT compared to no exercise. It is possible, that for healthy males, the effect of a single bout of exercise or exercise bouts separated by more than 24 h may not be enough stimulus to lower insulin responses to a glucose challenge.
KeywordsConsecutive days Exercise pattern Glucose Incremental area under the curve Insulin Single session
Area under the curve
Incremental area under the curve
Oral glucose tolerance test
Three alternate days
Three consecutive days
This study was supported in part by the Texas Woman's University Office of Research and Sponsored Programs, as well as the Department of Kinesiology. SED is currently supported by a T32 Postdoctoral Award from the National Institute of Diabetes and Digestive and Kidney Diseases (T32DK062710). The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIDDK or the NIH.
TC was responsible for collecting and analyzing data, and writing the manuscript. CI and RG were responsible for collecting data, analyzing data, and manuscript revisions. SD collected data and reviewed and edited the manuscript. MB, MO, and JR collected and analyzed data. AD reviewed data and edited manuscript. VB designed the research study, collected the data, and edited the manuscript.
Compliance with ethical standards
Conflict of interest
The authors have no conflicts of interest to declare.
- Cartee GD, Young DA, Sleeper MD, Zierath J, Wallberg-Henriksson H, Holloszy JO (1989) Prolonged increase in insulin-stimulated glucose transport in muscle after exercise. Am J Physiol 256:E494–E499Google Scholar
- Cohen J, Cohen P, West SG, Aiken LS (2015) Applied multiple regression/correlation analysis for the behavioral sciences. Routledge, New YorkGoogle Scholar
- Colberg SR, Sigal RJ, Fernhall B, Regensteiner JG, Blissmer BJ, Rubin RR, Chasan-Taber L, Albright AL, Braun B, American College of Sports Medicine, American Diabetes Association (2010) Exercise and type 2 diabetes: the American College of Sports Medicine and the American Diabetes Association: joint position statement executive summary. Diabetes Care 33(12):2692–2696. https://doi.org/10.2337/dc10-1548 CrossRefGoogle Scholar
- Jensen J, Aslesen R, Ivy JL, Brors O (1997) Role of glycogen concentration and epinephrine on glucose uptake in rat epitrochlearis muscle. Am J Physiol 272(4 Pt 1):E649–E655Google Scholar
- Newsom SA, Schenk S, Thomas KM, Harber MP, Knuth ND, Goldenberg N, Horowitz JF (2010) Energy deficit after exercise augments lipid mobilization but does not contribute to the exercise-induced increase in insulin sensitivity. J Appl Physiol (1985) 108(3):554–560. https://doi.org/10.1152/japplphysiol.01106.2009 CrossRefGoogle Scholar
- Riebe D, Ehrman JK, Liguori G, Magal M (2018) ACSM’s guidelines for exercise testing and prescription. Wolters Kluwer, Philadelphia, p 151Google Scholar
- Schell TC, Wright G, Martino P, Ryder J, Craig BW (1999) Postexercise glucose, insulin, and c-peptide responses to carbohydrate supplementation: running vs. resistance exercise. J Strength Cond Res 13(4):372–380Google Scholar
- Zorzano A, Balon TW, Goodman MN, Ruderman NB (1986) Additive effects of prior exercise and insulin on glucose and AIB uptake by rat muscle. Am J Physiol 251(1 Pt 1):E21–E26Google Scholar