European Journal of Applied Physiology

, Volume 119, Issue 5, pp 1137–1148 | Cite as

Effects of exercise in normobaric hypoxia on hemodynamics during muscle metaboreflex activation in normoxia

  • Gabriele Mulliri
  • Gianmarco Sainas
  • Sara Magnani
  • Silvana Roberto
  • Giovanna Ghiani
  • Mauro Mannoni
  • Virginia Pinna
  • Sarah J. Willis
  • Grégoire P. Millet
  • Azzurra Doneddu
  • Antonio CrisafulliEmail author
Original Article



Little is known about the cardiovascular effects of the transition from exercise in hypoxia (EH) to normoxia. This investigation aimed to assess hemodynamics during the metaboreflex elicited in normoxia after EH.


Ten trained athletes (four females and six males, age 35.6 ± 8.4 years) completed a cardiopulmonary test to determine the workload at anaerobic threshold (AT). On separate days, participants performed three randomly assigned exercise sessions (10 min pedalling at 80% of AT): (1) one in normoxia (EN); (2) one in normobaric hypoxia with FiO2 15.5% (EH15.5%); and (3) one in normobaric hypoxia with FiO2 13.5% (EH13.5%). After each session, the following protocol was randomly assigned: either (1) post-exercise muscle ischemia after cycling for 3 min, to study the metaboreflex, or (2) a control exercise recovery (CER) session, without any metaboreflex stimulation.


The main result were that both EH15.5% and EH13.5% impaired (p < 0.05) the ventricular filling rate response during the metaboreflex (− 18 ± 32 and − 20 ± 27 ml s−1), when compared to EN (+ 29 ± 32 ml s−1), thereby causing a reduction in stroke volume response (− 9.1 ± 3.2, − 10.6 ± 8.7, and + 5 ± 5.7 ml for EH15.5%, EH13.5% and EN test, respectively, p < 0.05). Moreover, systemic vascular resistance was increased after the EH15.5% and the EH13.5% in comparison with the EN test.


These data demonstrate that moderate exercise in hypoxia impairs the capacity to enhance venous return during the metaboreflex stimulated in normoxia. Overall, there is a functional shift from a flow to vasoconstriction-mediated mechanism for maintaining the target blood pressure during the metaboreflex.


Cardiac pre-load Venous return Stroke volume Cardiac output Blood pressure 



Anaerobic threshold


Control exercise recovery


Cardiac output


Cerebral tissue oxygenation


Cardiopulmonary test


Diastolic blood pressure


Diastolic time


Exercise in acute hypoxia


Exercise in normoxia


Heart rate


Mean blood pressure


Near-infrared spectroscopy


Nitric oxide


Post-exercise muscle ischemia


Pre-ejection period


Systolic blood pressure


Sympathetic nervous activity


Peripheral blood O2 saturation


Stroke volume/ventricular ejection time ratio


Stroke volume


Systemic vascular resistance


Mean ventricular ejection rate


Left ventricular ejection time


Ventricular filling rate


Carbon dioxide production


Maximum oxygen uptake


Maximum workload


Thoracic impedance



This study was supported by the University of Cagliari and the Italian Ministry of Scientific Research.

Author contributions

GM, AD and AC: conceived and designed research, conducted experiments, analysed data, and wrote the manuscript. GS, SM, SR, GG, MM, and VP: conducted experiments and analysed data. SJW and GPM: conceived and designed research, analysed data, and wrote the manuscript.

Compliance with ethical standards

Conflict of interest

The authors have no conflicts of interest that are directly relevant to the content of this manuscript.

Ethical approval

All the procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki Declaration and its later amendments or comparable ethical standards The study was approved by the ethics committee of the University of Cagliari.


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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2019

Authors and Affiliations

  • Gabriele Mulliri
    • 1
    • 2
  • Gianmarco Sainas
    • 1
  • Sara Magnani
    • 1
  • Silvana Roberto
    • 1
  • Giovanna Ghiani
    • 1
  • Mauro Mannoni
    • 1
  • Virginia Pinna
    • 1
    • 2
  • Sarah J. Willis
    • 3
  • Grégoire P. Millet
    • 3
  • Azzurra Doneddu
    • 1
  • Antonio Crisafulli
    • 1
    • 2
    Email author
  1. 1.Department of Medical Sciences and Public Health, Sports Physiology LabUniversity of CagliariCagliariItaly
  2. 2.International PhD in Innovation Sciences and TechnologiesUniversity of CagliariCagliariItaly
  3. 3.Institute of Sport Sciences, Faculty of Biology and MedicineUniversity of LausanneLausanneSwitzerland

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