Effects of chronic beetroot juice supplementation on maximum oxygen uptake, velocity associated with maximum oxygen uptake, and peak velocity in recreational runners: a double-blinded, randomized and crossover study
This study investigated the effects of chronic 3-day beetroot juice (BRJ) supplementation on maximum oxygen uptake (VO2max), velocity associated with VO2max(vVO2max), and peak velocity (Vpeak) in recreational runners.
Thirteen male recreational runners (age 28.2 ± 3.0 years, height 176.8 ± 0.1 cm, body mass 74.4 ± 9.5 kg) performed four tests on a treadmill in a randomized, double-blind, crossover design: two maximum incremental tests to determine VO2max and vVO2max, and two tests to determine Vpeak. Trials were performed following 3 days of supplementation of NO3−-rich BRJ in natura (8.4 mmol NO3− day− 1) or BRJ NO3−-depleted placebo (0.01 mmol NO3− day− 1), with the last dose being ingested 2 h before each test. During the tests, maximum heart rate (HRmax), maximal rating of perceived exertion (RPEmax), pre- and post-test glucose concentrations (Glucpre, Glucpost), and peak blood lactate concentration were determined.
VO2max was higher following BRJ vs PLA (46.6 ± 6.4 vs 45.1 ± 5.8 mL kg− 1 min− 1; P = 0.022), as well as vVO2max (14.5 ± 0.8 vs 13.9 ± 1.0 km h− 1P = 0.024) and Vpeak (15.5 ± 1.1 vs 15.2 ± 1.2 km h− 1P = 0.038), with no differences in the other variables.
Consumption of NO3−-rich BRJ in natura (8.4 mmol NO3− day− 1) once per day for 3 days improved VO2max, vVO2max and Vpeak in recreational runners without changing the other analyzed variables.
KeywordsNitrate supplementation Nitric oxide Running Exercise nutritional science Physical endurance
Blood lactate concentration
Peak blood lactate concentration
Beetroot juice supplementation
Maximum heart rate
Nitric oxide synthase
Rating of perceived exertion
Maximal rating of perceived exertion
Regulatory protein sirtuin
Number of seconds required to complete a stage
Number of seconds sustained during the incomplete stage
Running velocity of the last complete stage
Maximum oxygen uptake
Velocity associated with VO2max
We would like to thank the participants for their time and effort in this study.
All authors of this research paper have directly participated in the planning, execution, writing and analysis of this study. All authors of this paper have read and approved the final version submitted.
Compliance with ethical standards
Conflict of interest
The author(s) declare that they have no conflict of interest.
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