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European Journal of Applied Physiology

, Volume 119, Issue 2, pp 455–464 | Cite as

Tensiomyography parameters and serum biomarkers after eccentric exercise of the elbow flexors

  • Jan-Frieder HarmsenEmail author
  • Alexander Franz
  • Constantin Mayer
  • Christoph Zilkens
  • Bettina Alexandra Buhren
  • Holger Schrumpf
  • Rüdiger Krauspe
  • Michael Behringer
Original Article

Abstract

Purpose

The tensiomyography (TMG) technique is increasingly used to determine muscle contractile properties in exercise and injury management. The present study investigated the informative value of TMG parameters in correlation with commonly used (creatine kinase, CK; myoglobin, Mb) and novel candidate biomarkers of muscle damage (heart-type fatty acid-binding protein, h-FABP; high-mobility group box 1, HMGB1).

Methods

Ten untrained men performed 6 × 10 eccentric contractions of the elbow flexors at 110% of the concentric one repetition maximum. CK, Mb, h-FABP, HMGB1, arm circumference, pain and TMG data, including maximal displacement (Dm) and temporal outcomes as the contraction time (Tc), sustained time (Ts), delay time (Td), and relaxation time (Tr), were assessed pre-exercise, post-exercise, 20 min, 2 h and on the consecutive 3 days post-exercise.

Results

CK and h-FABP significantly increased beginning at 24 h, Mb already increased at 2 h (p < 0.05). HMGB1 was only increased immediately post-exercise (p = 0.02). Tc and Td remained unchanged, whereas Ts and Tr were significantly slower beginning at 24 h (p < 0.05). Dm was decreased within the first 24 h and after 72 h (p < 0.01). The % change from pre-exercise correlated for Dm with CK, Mb, and h-FABP the highest at 48 h (r = − 0.95, − 0.87 and − 0.79; p < 0.01) and for h-FABP with CK and Mb the highest at 24 h (r = 0.96 and 0.94, for all p < 0.001).

Conclusion

This study supports the correlation of TMG parameters with muscle damage markers after eccentric exercise. Therefore, TMG could represent a non-invasive and cost effective alternative to quantify the degree of muscle damage after exercise interventions.

Keywords

EIMD Creatine kinase Myoglobin h-FABP HMGB1 Nonresponder 

Abbreviations

ANOVA

Analysis of variance

ATP

Adenosine triphosphate

CK

Creatine kinase

Dm

Maximal displacement

EIMD

Exercise-induced muscle damage

h-FABP

Heart-type fatty acid binding protein

HMGB1

High-mobility group box 1

Mb

Myoglobin

MVC

Maximal voluntary contraction

NR

Nonresponders

POCT

Point-of-care-testing

TMG

Tensiomyography

Tc

Contraction time

Td

Delay time

Tr

Relaxation time

Ts

Sustained time

VAS

Visual analog scale

1-RM

One repetition maximum

Notes

Acknowledgements

The results of the study are presented clearly, honestly, and without fabrication, falsification, or inappropriate data manipulation. We thank TMG-BMC Ltd. (Ljubljana, Slovenia) for providing us a Tensiomyography device for the duration of the study.

Author contributions

JFH, AF, CM, CZ, RK, and MB contributed to the concept and design of the study; JFH, AF, and CM collected the data; JFH, AF, and CM conducted the experiments; BAB and HS were responsible for the laboratory analysis; JFH, AF, and MB analyzed and interpreted the data; JFH and AF created the figures; JFH, AF, and CM wrote the initial draft of the manuscript; and all authors reviewed the manuscript, commented critically, and approved the final version of the manuscript for submission.

Compliance with ethical standards

Conflict of interest

All authors declare that there are no financial and personal relationships with third parties or organizations that could have inappropriately influenced the present work. The authors further state that no funding was received.

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Copyright information

© Springer-Verlag GmbH Germany, part of Springer Nature 2018

Authors and Affiliations

  • Jan-Frieder Harmsen
    • 1
    Email author
  • Alexander Franz
    • 1
  • Constantin Mayer
    • 2
  • Christoph Zilkens
    • 1
  • Bettina Alexandra Buhren
    • 1
  • Holger Schrumpf
    • 1
  • Rüdiger Krauspe
    • 1
  • Michael Behringer
    • 3
  1. 1.Department of OrthopedicsUniversity Hospital DuesseldorfDuesseldorfGermany
  2. 2.Department of OrthopedicsUniversity Hospital EssenEssenGermany
  3. 3.Faculty of Sport SciencesGoethe University FrankfurtFrankfurtGermany

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